Abstract: FR-PO145

Different Effects of Aerobic and Combined Exercise on Mitochondrial OXPHOS Proteins in Skeletal Muscle of CKD Patients

Session Information

  • Mitochondriacs and More
    November 03, 2017 | Location: Hall H, Morial Convention Center
    Abstract Time: 10:00 AM - 10:00 AM

Category: Nutrition, Inflammation, and Metabolism

  • 1401 Nutrition, Inflammation, Metabolism

Authors

  • Gould, Douglas W., University of Leicester, Leicester, United Kingdom
  • Watson, Emma L., University of Leicester, Leicester, United Kingdom
  • Mcguire, Scott, Coventry university, Coventry, United Kingdom
  • Xenophontos, Soteris, University of Leicester, Leicester, United Kingdom
  • Wilkinson, Thomas James, University of Leicester, Leicester, United Kingdom
  • Graham-Brown, Matthew P.M., University of Leicester, Leicester, United Kingdom
  • Viana, Joao L., University Institute of Maia, Porto, Portugal
  • Smith, Alice C., University of Leicester, Leicester, United Kingdom
Background

Patients with CKD exhibit skeletal muscle wasting and dysfunction. Mitochondrial dysfunction is observed in non-dialysis (ND) CKD, resulting in abnormal oxidative phosphorylation (OXPHOS) and reduced exercise capacity. Exercise is a potent stimulus for mitochondrial adaptations, however the effects in CKD are under investigated.

Methods

17 ND-CKD patients (63±14 years; 11 female; eGFR: 28±8ml/min/kg/1.73m2) completed 12-weeks aerobic exercise (AE) (n=9), or combined aerobic and resistance exercise (CE) (n=8) 3x/wk. Muscle biopsies were obtained from the vastus lateralis at baseline (B1) and 24h (B2) post the first and final exercise sessions (B3). Mitochondrial OXPHOS proteins were analysed by Western blotting and reported as %change (90% CI) from baseline. Data was analysed using Cohens effect size (d) and magnitude-based inferences (MBI) that calculates quantitative and qualitative probabilities of a true effect based on effect and 90%CI.

Results

CE showed moderate effects on total OXPHOS protein expression with mean increases of 27% (-11 – 80) d=0.39 and 34% (-3 – 80) d=0.47 at B2 and B3 respectively. In comparison, the effects of AE were negligible with mean changes of +2% (-28 – 50) d=0.03 at B2 and -3% (-70 – 150) d=0.04 at B3. Figure 1 shows the results for the individual complexes along with MBI, where probability is the likelihood of a meaningful difference between time points in direction of change.

Conclusion

We report preliminary evidence of differential effects of AE and CE on OXPHOS complex protein expression in the muscle of ND-CKD. Mean % changes tended to be higher following CE, supported by larger effect sizes and MBI indicating beneficial effects for a number of respiratory chain complexes. This may suggest CE is more effective at modulating oxidative capacity of skeletal muscle in ND-CKD.

Magnitude based inference analysis for total OXPHOS and respiratory chain complexes (I-V)