ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005


The Latest on Twitter

Kidney Week

Abstract: TH-PO716

Study of mTORC2 Pathway on CD4+CD25+Treg in Diabetic Nephropathy

Session Information

Category: Diabetes

  • 501 Diabetes Mellitus and Obesity: Basic - Experimental


  • Li, Qiu yue, The First Affiliated Hospital of Nan Chang University, Nanchang, Jiangxi, China
  • Zhou, Jing, The first affiliated hospital of Nanchang University, Nanchang, Jiangxi, China

To investigate the effect of mTORC2 pathway blocker on CD4+CD25+Treg in diabetic nephropathy (DN) rats, and to explore the possible mechanism of Treg in DN podocyte injury.


Thirty SD rats were randomly divided into DN group, DN + FK506 group and DN + ku0063794 group.Treantment groups were respectively given FK506 1mg/kg/d, ku0063794 1mg/kg/d orally every day. Blood glucose, Creatinine clearance (Ccr) and urinary protein were measured at 0, 4 , 8 weeks. The expression of mTOR, Raptor, Rictor in renal tissue was detected at 4 , 8 weeks. The levels of IL-17 and TGF-β1 in peripheral blood were detected by ELISA, and the percentage of CD4+CD25+Treg was detected by flow cytometry at 4 , 8 weeks.


Urinary protein of model group was higher than control group(P<0.05), intervention groups were lower than model group (P<0.05); Ccr of model group compared with control group significantly decreased (P<0.05), intervention groups compared with model group significantly increased (P <0.05); mTOR, Raptor, Rictor increased significantly in the model group and decreased in the intervention groups (P<0.05); CD4+CD25+Treg of the model group was significantly lower than control group (P<0.05), and intervention groups were higher than model group (P<0.05); it of intervention groups at 8 week were higher than at 4 week (P<0.05); Pathological score of intervention groups compared with model group significantly decreased(P<0.05);TGF-β1 and IL-17 of the model group were significantly higher than control group (P <0.05), and the intervention groups were lower than model group (P<0.05); TGF-β1 of FK506 intervention group was lower than that of ku0063794 intervention group (P<0.05); Correlation analysis: In model group at 8 weeks, Rictor was negative correlation with CD4+ CD25=Treg and Ccr (P<0.05), positive correlation with TGF-β1,IL-17,urinary protein,pathological score (P<0.05); Raptor was positively correlated with urinary protein and pathological score (P<0.05), negative correlation with Ccr (P<0.05), no correlation with CD4+CD25+ Treg,TGF-β1,IL-17.


CD4+CD25+Treg may be regulated by the mTORC2 pathway in DN rats, and involved in the pathogenesis of DN.


  • Government Support - Non-U.S.