Abstract: TH-PO715

Effect of Dietary Fat/Carbohydrate Ratio on Renal Lipid Deposition in Rats with Diabetic Nephropathy

Session Information

Category: Diabetes

  • 501 Diabetes Mellitus and Obesity: Basic - Experimental

Authors

  • Sugimoto, Miho, Univ. of Shizuoka, Shizuoka, Japan
  • Yoshida, Takuya, Univ. of Shizuoka, Shizuoka, Japan
  • Ikegaya, Naoki, Yaizu City Hospital , Yaizu, Japan
  • Kumagai, Hiromichi, Univ. of Shizuoka, Shizuoka, Japan
Background

High-fat/low-carbohydrate diet (HFD) has been used to achieve glycemic control among diabetic patients. However, HFD may be harmful to those with diabetic nephropathy. This study aimed to examine the effect of HFD on diabetic nephropathy.

Methods

Twelve-week-old male Hos:ZFDM-Leprfa/fa (a novel diabetic rat strain) rats were maintained under a calorie-restrictive (60%–70% ad libitum) and isoenergetic condition with either HFD (14% protein, 40% fat, and 46% carbohydrate) or pair-fed control diet (14% protein, 15% fat, and 71% carbohydrate) for 7 weeks. Oral glucose tolerance test (OGTT), urinary protein excretion, creatinine clearance (Ccr), and renal triglyceride (TG) content were assessed at the end of the experiment. The renal histology and lipid deposition were also evaluated.

Results

The HFD rats had lower plasma glucose levels at 60 and 120 min OGTT than the control rats (at 120 min OGTT: 524±24 and 648±38 mg/dl for HFD and control rats, respectively; p<0.001). However, although the HFD rats had better glycemic control than the control rats, the former showed significantly lower Ccr (HFD and control rats: 1.63±0.13 and 3.86±0.34 L/day, respectively: p<0.001) and higher proteinuria than the latter. Furthermore, the HFD rats displayed significantly higher plasma and renal TG concentrations than the control rats. Glomerular mesangial expansion and lipid deposition in the proximal tubular cells were observed in the HFD rats. The renal TG content was correlated with the urinary liver-type fatty acid-binding protein excretion.

Conclusion

These results indicated that HFD may accelerate the progression of diabetic nephropathy, even under caloric restriction.

Funding

  • Government Support - Non-U.S.