Abstract: TH-PO403

Fish Oil Supplementation Reduces Inflammation but Does Not Restore Renal Function and Klotho Expression in an Adenine-Induced CKD Model

Session Information

Category: Nutrition, Inflammation, and Metabolism

  • 1401 Nutrition, Inflammation, Metabolism

Authors

  • Agudelo, Juan Sebastian, Universidade Federal de São Paulo, São Paulo, Brazil
  • Baia, Leandro Cunha, University Medical Center Groningen, Groningen, Netherlands
  • Ormanji, Milene Subtil, Universidade Federal de São Paulo, São Paulo, Brazil
  • Peixoto dos santos, Amandda rakell, Universidade Federal de São Paulo, São Paulo, Brazil
  • Camara, Niels OS, Universidade Federal de São Paulo, São Paulo, Brazil
  • Navis, Gerjan, University Medical Center Groningen, Groningen, Netherlands
  • De Borst, Martin H., University Medical Center Groningen, Groningen, Netherlands
  • Heilberg, Ita Pfeferman, Universidade Federal de São Paulo, São Paulo, Brazil
Background

CKD and inflammation promote loss of klotho expression. Given the well-established anti-inflammatory effects of n-3 fatty acids, we aimed to investigate the effect of fish oil supplementation in an experimental model of inflammatory renal damage.

Methods

Male C57BL/6 mice were fed an adenine-enriched diet (AD-10days) to induce inflammatory renal damage or standard chow (CTL) for 10 days, and in the subsequent 7 days received either fish oil (Post AD-Fish) or soybean oil (Post AD-Soy). Renal function, pro-inflammatory and profibrotic markers (picrosirius staining) were assessed and the expression of Klotho was evaluated by qPCR and Western-blot.

Results

When compared to CTL, the AD-10days group exhibited significantly higher mean serum creatinine (1.3±0.4 vs 0.8±0.1mg/dL), IL-6, CXCL10 and IL-1β (68.0±17.7 vs 1.0±0.2, 6.6±0.3 vs 1.0±0.2 & 3.5±1.5 vs 1.2±0.7, respectively), reduced renal klotho expression (0.2±0.0 vs 1.0±0.1), confirmed by Western-blot and a non-significant trend for increased fibrosis. As shown in the Figure, IL-6, CXCL9 and IL-1β were significantly decreased in Post-AD-Fish group but klotho expression was unaltered (also demonstrated by Western-blot). Serum creatinine and fibrosis did not differ statistically between Post-AD-Fish and Post-AD-Soy groups.

Conclusion

Fish oil supplementation reduced pro-inflammatory markers, but was not able to restore renal function or klotho expression in a model of inflammatory renal damage.