Abstract: SA-OR098

Liraglutide Treatment Improves Renal Vascular Function in Zucker Rats as Visualized by Microangiography

Session Information

Category: Hypertension

  • 1103 Vascular Biology and Dysfunction

Authors

  • Sukumaran, Vijayakumar, National Cerebral and Cardiovascular Center, Suita, Japan
  • Sonobe, Takashi, National Cerebral and Cardiovascular Centre, Osaka, Japan
  • Tsuchimochi, Hirotsugu, National Cerebral and Cardiovascular Centre, Osaka, Japan
  • Tatsumi, Eisuke, National Cerebral and Cardiovascular Center, Suita, Japan
  • Shirai, Mikiyasu, National Cerebral and Cardiovascular Center, Suita, Japan
  • Pearson, James T, National Cerebral and Cardiovascular Centre, Osaka, Japan
Background

Metabolic syndrome is a cluster of conditions that synergistically increase the risk of cardiovascular disease, type 2 diabetes, and premature mortality. In this present study, we investigated whether chronic liraglutide (LIRA) treatment affects the metabolic profile and renal vascular function in Zucker rats on a high-salt diet (6%NaCl).

Methods

Eight-week old Zucker lean and Zucker obese (fa/fa) rats were treated with vehicle or LIRA (0.1mg/kg/day) for 8 weeks. Glomerular filtration rate (GFR) was measured at 0 and 8 weeks by using fluorescein isothiocyanate (FITC)-sinistrin method in conscious rats. Further, we used X-ray microangiography to measure the renal arterial diameter (70-350 µm) and vessel number in the anaesthetised rats. Renal protein expression levels of nitrotyrosine and transforming growth factor (TGF)-beta 1 were detected by Western blotting.

Results

After 8 weeks of high-salt diet, systolic blood pressure was significantly increased, renal function and structure were impaired, and collagen deposition was abundant in renal tissues of vehicle-treated Zucker fa/fa rats. We found that in comparison to the untreated Zucker fa/fa rats, rats treated chronically with LIRA showed improved GFR and nitric oxide-mediated vasodilation in response to acetylcholine in small artery and arterioles (<200 µm diameter). Further, vessel internal diameter and visible vessel number (%) were greater in LIRA treated fa/fa rats compared to vehicle-treated rats (Figure 1). Moreover, LIRA treatment decreased the protein expression of nitrotyrosine and TGF-β1 compared to those of vehicle-treated rats.

Conclusion

These results suggest that LIRA treatment improved renal vascular function through dilation of small intrarenal arteries and arterioles.