Kidney Week

Abstract: SA-OR098

Liraglutide Treatment Improves Renal Vascular Function in Zucker Rats as Visualized by Microangiography

Session Information

• Vascular Biology and Dysfunction
  November 04, 2017 | Location: Room 394, Morial Convention Center
  Abstract Time: 05:54 PM - 06:06 PM

Category: Hypertension

• 1103 Vascular Biology and Dysfunction

Authors

• Sukumaran, Vijayakumar, National Cerebral and Cardiovascular Center, Suita, Japan

Vijayakumar Sukumaran, PhD



Currently, as a post-doctoral fellow (NCVC Research Institute, Japan), I am studying the renal microvascular function in rats with chronic kidney disease. Last year I have received the postdoctoral fellowship from the society of Japan promotion science (JSPS). In addition, I received research grant (NOVO NORDISK, Denmark) for project titles as “Protective roles of liraglutide on renal microvascular impairment in rats with diabetes mellitus by using synchrotron radiation microangiography”. I have published a total of 32 primary articles including diabetic nephropathy, chronic kidney disease and diabetes mellitus.

• Sonobe, Takashi, National Cerebral and Cardiovascular Centre, Osaka, Japan

Takashi Sonobe,

• Tsuchimochi, Hirotsugu, National Cerebral and Cardiovascular Centre, Osaka, Japan

Hirotsugu Tsuchimochi,

• Tatsumi, Eisuke, National Cerebral and Cardiovascular Center, Suita, Japan

Eisuke Tatsumi,

• Shirai, Mikiyasu, National Cerebral and Cardiovascular Center, Suita, Japan

Mikiyasu Shirai,

• Pearson, James T, National Cerebral and Cardiovascular Centre, Osaka, Japan

James T Pearson,

Background

Metabolic syndrome is a cluster of conditions that synergistically increase the risk of cardiovascular disease, type 2 diabetes, and premature mortality. In this present study, we investigated whether chronic liraglutide (LIRA) treatment affects the metabolic profile and renal vascular function in Zucker rats on a high-salt diet (6%NaCl).

Methods

Eight-week old Zucker lean and Zucker obese (fa/fa) rats were treated with vehicle or LIRA (0.1mg/kg/day) for 8 weeks. Glomerular filtration rate (GFR) was measured at 0 and 8 weeks by using fluorescein isothiocyanate (FITC)-sinistrin method in conscious rats. Further, we used X-ray microangiography to measure the renal arterial diameter (70-350 µm) and vessel number in the anaesthetised rats. Renal protein expression levels of nitrotyrosine and transforming growth factor (TGF)-beta 1 were detected by Western blotting.

Results

After 8 weeks of high-salt diet, systolic blood pressure was significantly increased, renal function and structure were impaired, and collagen deposition was abundant in renal tissues of vehicle-treated Zucker fa/fa rats. We found that in comparison to the untreated Zucker fa/fa rats, rats treated chronically with LIRA showed improved GFR and nitric oxide-mediated vasodilation in response to acetylcholine in small artery and arterioles (<200 µm diameter). Further, vessel internal diameter and visible vessel number (%) were greater in LIRA treated fa/fa rats compared to vehicle-treated rats (Figure 1). Moreover, LIRA treatment decreased the protein expression of nitrotyrosine and TGF-β1 compared to those of vehicle-treated rats.

Conclusion

These results suggest that LIRA treatment improved renal vascular function through dilation of small intrarenal arteries and arterioles.