Kidney Week

Abstract: FR-PO935

Deletion of the Gene for Adiponectin Accelerates Age-Related Kidney Injury

Session Information

• Geriatric Nephrology
  November 03, 2017 | Location: Hall H, Morial Convention Center
  Abstract Time: 10:00 AM - 10:00 AM

Category: Geriatric Nephrology

• 901 Geriatric Nephrology

Authors

• Bae, Eun Hui, Chonnam National University Hospital, Gwangju, Korea (the Republic of)

Eun Hui Bae,

• Choi, Hong sang, Chonnam national university hospital, Gwang-ju, Korea (the Republic of)

Hong sang Choi,

• Kim, Ha yeon, Chonnam National University Medical School, Dongku, Korea (the Republic of)

Ha yeon Kim,

• Kim, Chang Seong, Chonnam National University Hospital, Gwangju, Korea (the Republic of)

Chang Seong Kim,

• Ma, Seong Kwon, Chonnam National University Medical School, Dongku, Korea (the Republic of)

Seong Kwon Ma,

• Kim, Soo Wan, Chonnam National University Medical School, Dongku, Korea (the Republic of)

Soo Wan Kim,

Background

Aging causes renal fibrosis, and aging related renal changes are characterized by oxidative stress. However, the role of adiponectin in aging process has not been elucidated. The present study was aimed to investigate the role of adiponectin in renal fibrosis in aging.

Methods

We used male 2 and 12 months old C57BL/6 (wild type, WT) mice and adiponectin knock out (APN^-/-) mice. The protein expression of transforming growth factor β (TGF- β), Smad-2/3, Smad-4, Smad-6, α smooth muscle actin (α-SMA), collagen IV, pro-apoptoic Bax and anti-apoptotic protein Bcl-2, phosphorylated AMP-activated protein kinase (p-AMPK) was determined by semiquantitative immunoblotting. For the in vitro experiments, human proximal tubular epithelial (HK2) cells were treated with TGF- β with or without pretreatment of adiponectin.

Results

12 month old APN^-/- mice exhibited decreased body weight, increased albuminuria and kidney to body weight ratio compared to 12 months WT mice. Fibrosis markers such as α smooth muscle actin and collagen IV were increased. The protein expression of TGFβ, Smad-2/3, and Smad-4 was increased, while inhibitory Smad-6 decreased in 12 months APN^-/- mice compared to WT mice. ROS generation was also increased. Apoptosis marker such as Bax expression was increased while Bcl-2 expression was decreased in 12 months APN^-/- mice compared WT mice. Phosphorylation of AMP-activated protein kinase (AMPK) was decreased in 12 months APN^-/- mice compared to WT mice. TGFβ treatment showed decreased AMPK phosphorylation in HK2 cells. Pre-treatment of adiponectin attenuated fibrosis markers, apoptosis marker expression and ROS generation.

Conclusion

Our results suggest that adiponectin plays a role in the pathogenesis of progressive kidney injury associated with aging process.