Abstract: TH-PO400

Plant versus Animal Protein Improves Anti-Inflammatory Effects of HDL and Lessens CKD-Induced Atherosclerosis

Session Information

Category: Nutrition, Inflammation, and Metabolism

  • 1401 Nutrition, Inflammation, Metabolism

Authors

  • Kaseda, Ryohei, Niigata University, Niigata, Japan
  • Saito, Akihiko, Niigata University, Niigata, Japan
  • Hosojima, Michihiro, Niigata University, Niigata, Japan
  • Kuwahara, Shoji, Niigata University, Niigata, Japan
  • Kabasawa, Hideyuki, Niigata University, Niigata, Japan
  • Aoki, Hiroyuki, Niigata University, Niigata, Japan
  • Higuchi, Yuki, Niigata University, Niigata, Japan
  • Kon, Valentina, Vanderbilt University, Nashville, Tennessee, United States
  • Maruyama, Kentaro, Kameda Seika Co., Ltd, Niigata, Japan
  • Narita, Ichiei, Niigata University, Niigata, Japan
Background

Although CKD is known to cause endothelial injury that contributes to the many adverse consequences of CKD, few interventions specifically target endothelial cell injury. Little is known about the potential of nutritional effects on endothelial cell health, especially the impact of different dietary proteins. We and others have shown that in the CKD setting, HDL loses its anti-inflammatory effects, and even potentiates inflammation. Our aim was to determine whether differences in dietary protein source, namely animal protein versus plant protein can modulate renal injury-acceleration of atherosclerosis and anti-inflammatory properties of HDL.

Methods

12-week-old ApoE-deficient hyperlipidemic mice underwent uninephrectomy. The mice were pair-fed the usual casein-based diet (animal protein) or rice protein-based diet (plant protein extracted from rice endosperm by alkaline extraction method) for 6 weeks. We compared atherosclerotic lesions by en-face Sudan IV staining. HDL fraction was obtained by eliminating Apo B by polyethylene glycol precipitation. Human umbilical vein endothelial cells (HUVEC) were exposed to TNF-α together with HDL for 6 hours. Cellular expression of inflammatory markers (MCP-1, IL-6, and IL-1β) was assessed by real time RT-PCR.

Results

Atherosclerotic lesions were significantly reduced in rice protein-fed group compared to casein-fed mice (en-face atherosclerotic lesions 0.28±0.06 vs. 0.67±0.15mm2, p=0.038, N=5 and 5, respectively). HDL of rice protein-fed mice suppressed HUVEC’s inflammatory response compered to casein-fed mice (MCP-1, 3.83±0.73 vs. 7.42±0.39, p=0.003; IL-1β, 1.54±0.19 vs. 5.70±1.32 p=0.02; and IL-6, 0.52±0.07 vs. 0.98±0.06, p<0.001, N=5 and 5, respectively)

Conclusion

Plant protein-based diet significantly reduced kidney-injury driven atherosclerosis compared to animal protein-based diet. This anti-atherogenic effect is associated with anti-inflammatory effects of HDL. The results underscore the potential utility of nutritionally-based intervention in affecting atherosclerosis for CKD.

Funding

  • Private Foundation Support