Abstract: FR-PO116

The iTRAQ Technology Identifies Biomarkers for the Early Diagnosis of Contrast-Induced AKI

Session Information

Category: Acute Kidney Injury

  • 003 AKI: Clinical and Translational

Authors

  • Han, Lina, Ningbo NO.2 Hospital, Ningbo City, China
  • Luo, Qun, Ningbo NO.2 Hospital, Ningbo City, China
  • Zhou, Fangfang, Ningbo NO.2 Hospital, Ningbo City, China
  • Shen, Gen, Ningbo NO.2 Hospital, Ningbo, China
  • Ye, Honghua, Ningbo NO.2 Hospital, Ningbo, China
  • Wang, Zemin, Ningbo NO.2 Hospital, Ningbo, China
  • Li, Yumei, Ningbo NO.2 Hospital, Ningbo, China
Background

This study was to detect differentially expressed urine proteins in contrast-induced acute kidney injury (CI-AKI) patients after percutaneous coronary intervention(PCI) by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology, and to find new biomarkers for early diagnosis of CI-AKI.

Methods

We collected urine samples of older patients(>60yrs) before PCI and at 6hrs after PCI. And the samples were collected between July 1st 2015 and Jan 31st 2016 in Ningbo No.2 Hospital. Blood collected for biochemical analyses. iTRAQ technique was used to screen differentially expressed proteins, and to identify potential biomarkers by analysis of the biological process, cellular components, molecular functions, Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways and protein-protein interactions(PPI).

Results

48 older patients admitted for elective PCI were included in the study. Among these patients, 6 (4 male cases and 2 female cases) developed CI-AKI. In our study, the mean Scr levels were measured at 24h (121.80 ±54.31umol/L vs 94.43±33.56umol/L, P=0.059) and 48h (133.1±41.21umol/L vs 94.43 ±33.56umol/L, P=0.009) after PCI in the CI-AKI patients. Comparing to patients before PCI, a total of 151 proteins were identified in the CI-AKI group (6hrs after PCI). 74 proteins were up-regulated and 77 proteins were down-regulated. We identified 20 biological process, 20 cellular components, 20 molecular functions and 10 significant KEGG pathways. Combined with the PPI results, mannan-binding lectin (MBL)-associated serine protease 2 (MASP2), angiotensinogen (AGT) and apolipoproteins A-I(apoA-I) might play a role in the pathogenesis of CI-AKI.

Conclusion

1.The quantitative iTRAQ technology provided an accurate and effective assessment of identifying and profiling potential urine biomarkers for early diagnosis of CI-AKI in this study.2. Our research showed that MASP2, AGT, and apoA-I were significantly up-regulated at 6hr after PCI. And they were earlier than Scr for diagnosis of CI-AKI. They were potential urine biomarkers and played key roles in the pathogenesis of CI-AKI.

Funding

  • Government Support - Non-U.S.