Kidney Week

Abstract: TH-PO841

Epimorphin Expression in the Repair Process of Experimental Peritoneal Fibrosis in Mice

Session Information

• Peritoneal Dialysis - I
  November 02, 2017 | Location: Hall H, Morial Convention Center
  Abstract Time: 10:00 AM - 10:00 AM

Category: Dialysis

• 608 Peritoneal Dialysis

Authors

• Yamada, Muneharu, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Muneharu Yamada,

• Komatsu, Shuuhei, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Shuuhei Komatsu,

• Kojima, Aki, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Aki Kojima,

• Oshima, Taito, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Taito Oshima,

• Hirose, Go, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Go Hirose,

• Kojima, Tadasu, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Tadasu Kojima,

• Sugisaki, Kentaro, Hachioji Medical Center, Tokyo, Japan

Kentaro Sugisaki,

• Tomiyasu, Tomohiro, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Tomohiro Tomiyasu,

• Yoshikawa, Noriko, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Noriko Yoshikawa,

• Oda, Takashi, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan

Takashi Oda,

Background

Epimorphin is a mesenchymal cell surface-associated protein that is essential for epithelial morphogenesis in embryonic organs. However, recent studies indicate important roles of epimorphin in the repair process from pathologic organ damages, such as pulmonary fibrosis and liver injury. We previously reported that epimorphin was involved in the repair of fibrosis in UUO release mice (Lab Invest 2010). In the previous Meeting, we presented the increased epimorphin expressions in the submethothelial area corresponding to the fibrotic area in mice model of peritoneal fibrosis. In the present study, we evaluated the expression of epimorphin in the repair of peritoneal fibrosis.

Methods

Peritoneal fibrosis was induced by the injection of 0.1% chlorhexidine gluconate in 15% ethanol and 85% normal saline (CG-injected mice) into peritoneal cavity of 10 week-old male C57/Bl6 mice every other day for three weeks. As the repair phase of peritoneal fibrosis mice model, we used peritoneal tissues of the CG-injected mice which were harvested at 1,2,3,7 and 14 days after the last CG injection. Morphologic peritoneal changes were assessed by Masson’s Trichrome staining. Epimorphin expressions were assessed by immunohistochemically and real-time RT-PCR.

Results

In the repair of CG-injected mice, the thickening of the submesothelial compact zone was decreased over time in Masson’s trichrome staining. However epimorphin expression was increased at 2 days rather than at 1 day after the last CG injection, and significantly decreased at 3, 7 and 14 days compared to 2days after the last CG injection by real-time RT-PCR.

Conclusion

These findings suggest that epimorphin may have important role in the repair of peritoneal fibrosis similar to that of UUO release model in mice as reported previously.