Abstract: FR-PO665

The Expression of Serum lncRNA GAS5 and miR-21 ceRNA Associated with Clinical and Pathological Changes in Patients with Diabetes and Diabetic Nephropathy

Session Information

Category: Diabetes

  • 502 Diabetes Mellitus and Obesity: Clinical

Author

  • Fan, Qiuling, Department of Nephrology, The First Hospital, China Medical University, Shenyang, China
Background

To analyze the expression of serum lncRNA GAS5 and miR-21 in patients with diabetes mellitus and diabetic nephropathy, and to analyze the correlation between the expression of them and the clinical and pathlogical parameters, and to testify the function of them in the pathogenesis of diabetes mellitus and diabetic nephropathy, in order to find novel therapeutic targets and biomarkers.

Methods

The patients were divided into three groups, diabetic nephropathy group: patients proven by renal biopsy, diabetes group: patients with diabetes of normal Urine albumin creatinine ratio , normal control group. The expression of lncRNA GAS5 and miR-21 in serum samples were detected by real-time quantitative PCR. The correlation of serum lncRNA GAS5 and miR-21 expression with the clinical parameters were analyzed.

Results

The expression of serum lncRNA GAS5 was significantly down-regulated and the expression of serum miR-21 was significantly up-regulatedin in diabetes mellitus and diabetic nephropathy patients comparing with the normal control group.The expression of serum miR-21 was gradually up-regulated (P=0.038) along with the progression of renal biopsy stage IIb-IV of DN. FBG and HbA1cwere significantly negatively correlated with serum lncRNA GAS5, and FBG was independently correlated with serum lncRNA GAS5. urine microalbumin, TC, Cr, Urea(r=0.516,P<0.001) and SBP were significantly positively correlated with serum miR-21. ALB and eGFRwere significantly negativly correlated with serum miR-21, and ALB was independently correlated with serum miR-21.The diagnostic efficiency of serum lncRNA GAS5, miR-21 and lncRNA GAS5/miR-21 as "diagnostic signature" for DM were good, for lncRNA GAS5, AUC=0.7302, p=0.03, cutoff point was 0.0056, sensitivity was 62.86%, specificity was 77.78%; for miR-21, AUC=0.8397, p=0.002, cutoff point was 0.6600, sensitivity was 77.14%, specificity was 77.78%. The diagnostic efficiency of serum miR-21 and lncRNA GAS5/miR-21 as "diagnostic signature" for DN were good, for miR-21, AUC=0.9179, p<0.0001, cutoff point was 0.9900, sensitivity was 76.00%, specificity was 94.74%.

Conclusion

Serum lncRNA GAS5 and miR-21 expression can be used as noninvasive diagnostic marker for diabetes and diabetic nephropathy.