Abstract: TH-PO114

Clinical Significance of Urinary Podocyte-Derived Microparticle Detection in Idiopathic Membranous Nephropathy

Session Information

Category: Glomerular

  • 1004 Clinical/Diagnostic Renal Pathology and Lab Medicine

Authors

  • Lu, Jian, Zhongda Hospital, Southeast University Medical School, Nanjing City, JIANGSU , China
  • Ma, Kun ling, Zhongda Hospital, Southeast University Medical School, Nanjing City, JIANGSU , China
  • Zhang, Yang, Zhongda Hospital, Southeast University Medical School, Nanjing City, JIANGSU , China
  • Hu, Zebo, Zhongda Hospital, Southeast University Medical School, Nanjing City, JIANGSU , China
Background

Microparticles (MPs) are a type of extracellular vesicles (EVs) shed from the outward budding of cytoplasma membranes during cell apoptosis and/or activation. These microsized particles release specific contents (lipids, proteins, microRNAs, etc.) and are active participants in a wide range of both physiological and pathological processes at molecular levels. This study aimed to observe the change of urinary podocyte-derived microparticle level and to explore its potential clinical significance in idiopathic membranous nephropathy(IMN).

Methods

We prospectively enrolled patients with IMN (n = 24) before initial Immunosuppressive therapy to study renal tissue pathology using Periodic acid–Schiff staining by light microscope and ultrastructural observation by transmission electron microscope. After isolation from urine samples, podocalyxin-positive podocyte-derived microparticles were characterized by flow cytometry. Twenty IMN patients were studied again 6 months after Glucocorticoids in combination with tacrolimus intervetion. Health Volunteers (n= 15) served as controls. The correlation of urinary podocyte microparticles and clinical and pathological factors in IMN patients was analyzed.

Results

Compared with the control group, there were increased serum PL2AR antibody tite and 24-hour urinary protein (all P<0.05). The fraction of podocyte microparticles among urinary microparticles was elevated in IMN compared with health volunteers (P<0.01) and decreased after 6 months therapy and after controlling for clinical parameters.


Conclusion

The excretion of urine podocyte-derived microparticles might reflect podocyte injury and might be closely associated with the progression of IMN.