Abstract: FR-PO722

Chemokine Receptor 8 in Peripheral Blood Mononuclear Cells Can Distinguish Active ANCA-Associated Vasculitis from Infectious Complications

Session Information

Category: Glomerular

  • 1004 Clinical/Diagnostic Renal Pathology and Lab Medicine

Authors

  • Sanada, Satoru, Japan Community Health Care Organization Sendai Hospital, Sendai, Japan
  • Akiyama, Yukako, Tohoku university, Sendai Miyagi, Japan
  • Sato, Mitsuhiro, Japan Community Health Care Organization Sendai Hospital, Sendai, Japan
  • Sato, Toshinobu, Japan Community Health Care Organization Sendai Hospital, Sendai, Japan
  • Taguma, Yoshio, Japan Community Health Care Organization Sendai Hospital, Sendai, Japan
Background

Infectious complications are major causes of death in ANCA-associated vasculitis (AAV). Similar clinical symptoms between AAV and infection may cause diagnostic difficulty despite totally opposite treatment in two situations. Aim of this study is to identify a new biomarker for AAV, which enables to distinguish vasculitis and infections.

Methods

Peripheral blood mononuclear cells (PBMC) were collected from 222 patients with AAV, including patients in active, remission and infectious complications and patients with other rapidly progressive glomerulonephritis. Chemokine receptor 8 (CCR8) was assessed using quantitative PCR and flow cytometry. Quality of mRNA was measured by bioanalyzer before reverse transcription to provide reproducible results.

Results

CCR8 mRNA level in PBMC was significantly higher in patients with MPO-ANCA vasculitis compared to that in healthy control, which was confirmed by upregulated CCR8 protein expression in FACS. The area under ROC curve was 0.923 (95%CI: 0.842-1.000) with a sensitivity of 87.5% and a specificity of 100%. Lupus nephritis nor purpura nephritis did not show high CCR8 mRNA levels. Among MPO-ANCA vasculitis, CCR8 mRNA levels in patients with remission and patients with infectious complications during remission were lower compared to that in patients with active. The area under ROC curve was 0.924 (95%CI: 0.846-1.000) compared with active AAV and infectious complication.

Conclusion

CCR8 mRNA level in PBMC was associated with AAV activity, however, infectious complications did not affect CCR8 expression, suggesting that CCR8 could be a useful diagnosis biomarker for AAV.

Funding

  • Private Foundation Support