Abstract: FR-PO971

The Differential Expression of Circular RNAs in Exosomes from Serum and Urine in Patients with IMN

Session Information

Category: Bioengineering and Informatics

  • 101 Bioengineering and Informatics

Authors

  • Ma, Hualin, Shenzhen People's Hospital, Shenzhen, China
  • Zhang, Xin-zhou, Shenzhen People's Hospital, Shenzhen, China
Background

To further explore the pathogenesis of idiopathic membranous nephropathy (IMN), the technique of gene-sequencing was used to analyze the differentially expressed circRNAs in exosomes from both the serum and urine of patients with IMN, which may lay the foundation for the research of circRNAs as a new class of exosome-based idiopathic membranous nephropathy diagnosis biomarkers.

Methods

Ten patients with idiopathic membranous nephropathy (IMN group) and ten normal controls (NC group) were recruited as experimental subjects in our study. The exosomes were extracted from the collected serum and urine by the ExoQuick Exosome Precipitation Solution and ultracentrifugation. Then, the pure circRNAs were extracted from the exosomes with a series of enzymatic reactions. Afterwards, the significantly differentially expressed circRNAs were chosen by the method of gene-sequencing to analyze the function of corresponding target genes.

Results

Compared with normal controls, the circRNAs were reduced in the exosomes from serum of patients with IMN, which mostly originated from intron gene regions. Meanwhile, a total of 89 circRNAs were significantly differentially expressed, which were also mostly derived from intron gene regions, including 49 up-regulated and 40 down-regulated genes. However, the species were increased in the exosomes from the urine of patients with IMN compared to normal controls, and they mainly originated from exon gene regions. Simultaneously, a total of 60 circRNAs were significantly differentially expressed, which primarily belonged to intron gene regions, including 54 up-regulated and 6 down-regulated regions. Compared with the circRNAs detected from urinary exosomes, a total of 59 circRNAs were significantly differentially expressed in the exosomes from the serum of patients with IMN, which also mostly originated from intron gene regions, including 32 up-regulated and 27 down-regulated regions.

Conclusion

The significant differential and specific expression of circRNAs in the exosomes from the serum and urine of patients with IMN were observed. For example, MUC3A, which originated from chr7:100550808|100551062, could be considered a potential diagnostic biomarker of IMN. Furthermore, these figures suggested that the significantly differentially expressed circRNAs can be used as a reference or supplement in the research of the pathogenesis of IMN.