Abstract: FR-PO622

High Intensity Interval Training (HIIT) Attenuates Proteinuria and Improves Physical Capacity in STZ Diabetic Rats

Session Information

Category: Diabetes

  • 501 Diabetes Mellitus and Obesity: Basic - Experimental

Authors

  • Reinecke, Natalia, UNIFESP, Sao Paulo, Brazil
  • Luiz, Rafael, None, São Paulo, SÃO PAULO, Brazil
  • Saud, Alexandre, UNIFESP, Sao Paulo, Brazil
  • Rampaso, Rodolfo Rosseto, None, São Paulo, SÃO PAULO, Brazil
  • Silva, Wesley, Universidade Federal de São Paulo, Sao Paulo [SP], Brazil
  • Filho, Samuel Trindade, Universidade Federal de São Paulo, Sao Paulo [SP], Brazil
  • Almeida, Waldemar S., EPM/UNIFESP, São Paulo, Brazil
  • Schor, Nestor, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
Background

The insertion of Diabetic patients on a physical training program is a challenge, given that ‘lack of time’ is one of the most cited barriers. HIIT has demonstrated to have several benefits to these subjects, even requiring a lower commitment time than current recommendation of exercise for this population. The purpose of this investigation was to examine the effects of HIIT on renal function and physical capacity in STZ Diabetic rats and compare these results with the effects of endurance moderated exercise.

Methods

Wistar rats were divided into four groups 6-8 animals: Sedentary Control(SC), Sedentary Diabetic(SD), Diabetic Endurance(DE) and Diabetic HIIT(DH). DH group were submitted to following protocol: 10bursts of 1min(90% of maximal test) intersected with 10 periods of low intensity walking(40% of maximal test) 3days/wk, 8weeks total. Endurance training consisted of 60min of moderated exercise(60% of maximal test) 5days/wk, 8weeks total. Diabetes was induced by a single STZ injection(50mg/kg i.v.). At the end of protocol animals underwent to an Ergospirometry test to detect the peak of oxygen consumption(VO2peak).

Results

HIIT improved Exercise Capacity(VO2peak:48±16 vs 32.8±7ml/kg/min, DH vs SD,p<0.05) as well as Endurance training(VO2peak:53.1±11 vs 32.8±7ml/kg/min, DE vs SD,p<0.05). HIIT, but not Endurance training, attenuated the increase in proteinuria caused by Diabetes in comparison to SD(9.9±6 vs 19.6±1.9mg/dL/24h, p<0.05, respectively). Both training attenuated the increase in urinary volume, water ingestion, Serum Urea and partially prevent low Creatinine Clearance(CrCl) when compared to SD.

Conclusion

Results of this study show that HIIT can improve exercise capacity, partially prevent low CrCl and attenuate the increase in proteinuria and in other diabetic symptoms as polyuria and polydipsia. These data suggests that HIIT can be a time-efficient adjunct treatment to minimize Diabetes complications.

 SCSDDEDH
Glycemia(mg/dl)112±7460±70*500±26*465±51*
Urinary Volume(ml)14±595±14 *118±18*93±24*#&
H2O Consumption(ml)18±5124±22*146±18*93±24*#&
CrCl(ml/dl)0.8±0.10.4±0.2*0.7±0.30.6±0.02
Ser Urea(mg/dl)41±872±14*55±16#54±9#

*p<0.05vsSC; #p<0.05vsSD, &p<0.05vsDE

Funding

  • Government Support - Non-U.S.