Kidney Week

Abstract: TH-OR126

Normothermic Ex-Vivo Kidney Perfusion Improves Function of Marginal Renal Grafts That Were Subjected to Prolonged Ischemia prior to Preservation

Session Information

• Transplantation: Top 10 Basic and Experimental Abstracts
  November 02, 2017 | Location: Room 295, Morial Convention Center
  Abstract Time: 06:06 PM - 06:18 PM

Category: Transplantation

• 1701 Transplantation: Basic and Experimental

Authors

• Urbanellis, Peter, University Health Network, Toronto, Ontario, Canada

Peter Urbanellis, MD, MS

• Hamar, Matyas, University Health Network, Toronto, Ontario, Canada

Matyas Hamar,

• Linares, Ivan, University Health Network, Toronto, Ontario, Canada

Ivan Linares,

• Kollmann, Dagmar, University Health Network, Toronto, Ontario, Canada

Dagmar Kollmann,

• Ganesh, Sujani, University Health Network, Toronto, Ontario, Canada

Sujani Ganesh,

• Yip, Paul M., University Health Network, Toronto, Ontario, Canada

Paul M. Yip,

• John, Rohan, University Health Network, Toronto, Ontario, Canada

Rohan John,

• Mucsi, Istvan, University Health Network, Toronto, Ontario, Canada

Istvan Mucsi,

• Konvalinka, Ana, University Health Network, Toronto, Ontario, Canada

Ana Konvalinka,

• Bagli, Darius, The Hospital for Sick Children, Toronto, Ontario, Canada

Darius Bagli,

• Grant, David, University Health Network, Toronto, Ontario, Canada

David Grant,

• Robinson, Lisa, The Hospital for Sick Children, Toronto, Ontario, Canada

Lisa Robinson,

• Selzner, Markus, University Health Network, Toronto, Ontario, Canada

Markus Selzner,

Background

Normothermic ex-vivo kidney perfusion (NEVKP) is an emerging technique for renal graft preservation. We investigated whether NEVKP could promote improved marginal graft function compared to cold storage in a model of donation after cardiac death.

Methods

Kidneys from 30kg Yorkshire pigs were removed following 30, 60, 90, or 120 minutes of warm ischemia (WI). These grafts were then preserved in either cold histidine-tryptophan-ketoglutarate solution (CS) or subjected to pressure-controlled NEVKP for 8 hours prior to heterotopic autotransplantation.

Results

Prolonging WI time prior to kidney retrieval and subsequent storage in CS resulted in grafts that demonstrated incremental posttransplant increases in serum creatinine with grafts subjected to 120min of WI having persistent elevation (POD7: 13.45±3.50mg/dl vs baseline: 1.1±0.33mg/dl p<0.01, n=4). During NEVKP perfusion, 120min WI grafts cleared lactate from perfusion solution (0hr: 10.48±0.93mmol/L vs 7hr: 1.48±0.85mmol/L, p<0.01, n=5), had decreasing intra-renal resistance (0hr: 2.26±0.9mmHg/mL/min vs 7hr: 0.37±0.6mmHg/mL/min, p<0.01), and continuous urine production. Posttransplantation, 120min WI grafts with NEVKP, compared to CS, demonstrated significantly decreased serum creatinine peak values (POD4: 12.62±2.34mg/dl vs POD5: 18.95±1.11mg/dL, p=0.001) and higher creatinine clearance (POD4: 6.61±4.03mL/min vs 0.35±0.30mL/min, p=0.02 and POD7: 26.31±11.54mL/min vs 9.78±4.6mL/min, p=0.03). On POD7, serum creatinine returned to baseline values in the NEVKP group (POD7: 4.88±5.57mg/dL vs baseline: 1.02±0.16mg/dL, p=0.16) but not the CS group (POD7: 13.45±3.50mg/dl vs baseline: 1.1±0.33mg/dl p<0.01, n=4). Histology from 120min WI NEVKP grafts at POD7 demonstrated decreased tubular injury scores compared to cold CS grafts (1.8+/-0.8 vs. 3.0+/-0.0, p=0.03) as assessed by a blinded pathologist.

Conclusion

Kidney grafts subjected to 120min of WI before retrieval showed significant improvement in function following 8hrs of continuous pressure-controlled NEVKP compared to CS. This suggests NEVKP could be utilized to expand the donor pool through the consideration of extreme marginal grafts for transplantation.

Funding

• Government Support - Non-U.S.