Abstract: SA-PO337
Four Weeks of Resistance Training (RT) Improves Physical Capacity, Creatinine Clearance, and Glomerulosclerosis and Decreases Mortality Rate in Rats with CKD
Session Information
- Mechanisms Associated with Kidney Fibrosis - II
November 04, 2017 | Location: Hall H, Morial Convention Center
Abstract Time: 10:00 AM - 10:00 AM
Category: Chronic Kidney Disease (Non-Dialysis)
- 308 CKD: Mechanisms of Tubulointerstitial Fibrosis
Authors
- Luiz, Rafael, UNIFESP, SAO PAULO, SAO PAULO, Brazil
- Saud, Alexandre, UNIFESP, SAO PAULO, SAO PAULO, Brazil
- Silva, Wesley, UNIFESP, SAO PAULO, SAO PAULO, Brazil
- Gloria, Maria A., UNIFESP, SAO PAULO, SAO PAULO, Brazil
- Rampaso, Rodolfo Rosseto, UNIFESP, SAO PAULO, SAO PAULO, Brazil
- Pessoa, Edson Andrade, UNIFESP, SAO PAULO, SAO PAULO, Brazil
- Schor, Nestor, UNIFESP, SAO PAULO, SAO PAULO, Brazil
Background
The aim of this study was to evaluate if 4 weeks of RT improves physical capacity (strength gain and VO2peak), renal function, glomerulosclerosis and mortality in rats with CKD by nephrectomy 5/6 (Nx5/6).
Methods
Adult Wistar rats were divided in four groups (n=8): Sedentary (S) Exercise (E), Nx 5/6 + Sedentary (NSR), Nx 5/6 + Exercise (NER). We evaluated creatinine clearance (CrCl), proteinuria (uProt), blood urea nitrogen (BUN), glomerulosclerosis, mean arterial pressure (MAP) as well mortality rate. EXE periods were as follows: 6 to 12 climbs/day, 5 days a week, during 4 weeks, 40 to 60% of maximal load test (MLT). The physical capacity was performed with maximal load test (MLT), ergoespirometry test (Vo2peak) and maximal exercise test (MEtest).
Results
The CrCl was improve in NER (43%) vs NS group, (p<0.05). Proteinuria was different in NSR and NER vs S and R groups but not in NSR vs NER. BUN was higher in NSR and NER vs S and R. Glomerulosclerosis was different in NSR vs NER (p<0.05). The MAP was lower in NER vs NSR group (p<0.05). Physical Capacity (MLT, VO2peak and Mtest) was increased in NER vs NSR. A higher mortality rate was observed in NS (30%).
Conclusion
Results suggested that the 4 weeks of RT minimize the impact of 5/6Nx by increase in physical capacity (MLT, VO2peak and Mtest), reduce the impact on CrCl (43%) and improve in glomerulosclerosis (44%). These parameters indicate that exercise could have a protective effect, especially under this experimental protocol. Thus, this study suggests that the exercise plays a preventive role in mortality and could be an additional strategy to be employed in CKD.
| S | R | NSR | NER | |
| Weight (g) | 363±14 | 346±8 | 312±13 | 325±17 |
| CrCl (ml/min) | 1.6±0.1 | 2.2±0.2 | 0.3±0.1*# | 0.7±0.2*# |
| uProt (mg/24hrs) | 6.8±0.9 | 6.8±0.5 | 38.1±3.0 | 37.6±3.4 |
| BUN (mg/dL) | 42.6±2.7 | 42.2±2.6 | 158.5±20.1 | 130.7±13.5 |
| Glomerulosclerosis (%) | 0 | 0 | 2.8±0.4 & | 1.6±0.4 *# |
| MAP (mmHg) | 132±3 | 126±2 | 213±5 & | 180±10 |
| Vo2 peak (ml/kg/min) | 33.8±0.9 | 40.0±1.5 * | 30.9±0.2 # | 40.7±0.6 * % |
| MLT/Weight (g) | 1.3±0.1 | 1.7±0.1 * | 1.2±0.0 # | 1.5±0.6 % |
| Mtest (m/min) | 37±2 | 43±1 | 33±2 & | 38±1 |
| Mortality Rate (%) | 0 | 0 | 30 | 10 |
* vs S; # vs R; % vs NSR and & vs all.