Abstract: FR-PO255

Adipose Tissue-Derived Stem Cells (ASC) Reverses Kidney Disease Progression in SHR Rats Induced to Metabolic Syndrome

Session Information

  • Stem Cells
    November 03, 2017 | Location: Hall H, Morial Convention Center
    Abstract Time: 10:00 AM - 10:00 AM

Category: Developmental Biology and Inherited Kidney Diseases

  • 402 Stem Cells

Authors

  • Batista, Marcelo Costa, UNIFESP, São Paulo, São PAULO, Brazil
  • Nakamichi, Renata, Universidade Federal de São Paulo, Santo André, Brazil
  • Oliveira, Camila Nunes, UNIFESP, São Paulo, São PAULO, Brazil
  • Cesaretti, Mario luis Ribeiro, Universidade Federal de São Paulo, Santo André, Brazil
  • Dalboni, Maria, Universidade Federal de São Paulo, Santo André, Brazil
  • Quinto, Beata Marie redublo, UNIFESP, São Paulo, São PAULO, Brazil
Background

Visceral obesity, the physiopathological basis of Metabolic Syndrome (MS), determines a set of metabolic abnormalities linked to increased risk of kidney disease in the overall population. The excessive expansion of visceral adipose tissue, resulting in hypertrophied adipocytes is implicated in the development of hypoxic environment and increases inflammatory proteins. The adipose tissue is also considered an important source of stem cells which are able to proliferate and differentiate into multiple cell lines reducing the expression of inflammatory proteins.
The aim of the study is to evaluate the treatment of the kidney disease progression in SHR rats induced to MS with ASCs.

Methods

SHR rats were induced to MS by hyperlipid/hypercaloric diet for 12 weeks, and then treated with injection of 2x105 ASC for 1 and 2 weeks, respectively. After this period, rats were sacrificed for analysis of albuminuria, as well as kidney function (serum concentration of creatinine and Cystatin C, and urinary NGAL) for comparison with baseline parameters. The characterization of ASC extracted from subcutaneous tissue of SHR control rats was performed through flow cytometry method.

Results

Previous analysis has shown that ASC expressed the cell surfaces markers: CD34, CD35, CD90 and CD105, confirming its characterization in adipocyte. Our results demonstrated a statistically significant deterioration of lipid profile, as shown by decrease in HDL-c and increase in triglycerides and total cholesterol, on those animals induced to MS when compared with the control group (CT). This adverse lipid profile was reversed on those MS-induced animals treated with stem cells. Parallel with the induction of MS, we observed the development of kidney disease as shown by the enhancement in albuminuria as well as an increase in creatinine, Cystatin C and Ngal concentrations among those rats induced to MS. Similarly with lipid profile, we could also evidence a reversion of kidney disease progression through the improvement in the above mentioned kidney disease parameters, among the MS-Induced rats treated with adipose tissue-derived stem cells.

Conclusion

Adipose tissue-derived stem cells reversed Metabolic Syndrome related kidney disease progression in SHR rats.

Funding

  • Private Foundation Support