Abstract: FR-PO612

Aerobic Exercise Training Reduces Renal Inflammatory Factors, Fibrosis, and Proteinuria in Diabetic Rats

Session Information

Category: Diabetes

  • 501 Diabetes Mellitus and Obesity: Basic - Experimental

Authors

  • Rampaso, Rodolfo Rosseto, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
  • Luiz, Rafael, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
  • Reinecke, Natalia, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
  • Pessoa, Edson Andrade, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
  • Silva, Kleiton Augusto Santos, University of Missouri, Columbia, Alabama, United States
  • Jorge, Luciana, None, São Paulo, SÃO PAULO, Brazil
  • Gloria, Maria A., Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
  • Schor, Nestor, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
Background

The aim of this study was to evaluate the role of aerobic exercise in controlling the progression of diabetic nephropathy as proteinuria, fibrosis, inflammatory factors, and thus, its possible renoprotective effects

Methods

Adult male Wistar rats divided into 4 groups: Sedentary controls, (SED, n=8), Diabetes+Sedentary (DM-SED, n=8), Diabetes+Exercise (DM-EXE, n=8) and Exercise+Controls (EXE, n=8). DM was induced with streptozotocin (STZ), 50mg/kg i.v. The physical training was done on treadmill 60 min/day, 5 days a week for 8 weeks. Weekly it was determined the Maximal Exercise Test (set at 65-70% of MEtest). Fibrosis (m2), Glycemia 24h post training (glycemiapt), MEtest, creatinine clearance/BW (CrCl/BW), mean arterial pressure (MAP), proteinuria (uProt), renal inflammatory factors (IL-6,IL-10 and TNF-alpha) were measured

Conclusion

Reductions in glycemia, fibrosis and MAP comparing DM-EXE vs DM-SED. The DM-EXE controlled weight loss compared to DM-SED, but did not prevent alteration in the CrCl/BW. However, the effect of the EXE was strikingly observed in the reduction in both, mean uProt excretion (60% and 25%) and in inflammatory factors comparing DM-SED vs. DM-EXE. Therefore, preliminary data suggest that aerobic exercise can reduce proteinuria, renal fibrosis and inflammatory factors in diabetic animals and consequently diminish potential effects caused by diabetic and potentially could reduce the progression to renal failure

 SED

DM-SED

DM-EXE
EXE
uProt ( mg/24h)
17 ± 0.88
46 ± 2.05 *#&
18 ± 0.72

16 ± 0.99
IL-6 (pg/ml)
541 ± 98
993 ± 40*#&
768 ± 74*&
391 ± 22

IL-10 (pg/ml)
545 ± 86
876 ± 34*#&
654 ± 31*&
453 ± 28

TNF-alpha (pg/ml)

3.05 ± 0.4
5.18 ± 0.76*#&
4.08 ± 0.22*&
2.32 ± 0.51
Fibrosis (m2)
5.87±0.92
11.42±1.84*#&
8.37±2.13*
7.47±1.27
CrCL/ (ml/min/BW)
5.65 ± 0.66

5.02 ± 0.43
4.19 ± 0.37
4.21 ± 0.29
glycemiapt (mg/dl)
103 ± 2.03
551 ± 7.03*&
491 ± 5.50*&
83 ± 2.57
MAP (mmHg)
122 ± 1.89
133.88 ± 1.79*#&
122 ± 1.35
121 ± 2.11
Weight (g)
455 ± 6.00
236 ± 14.41*#&
324 ± 9.34*&
387 ± 8.71
MEtest (m/min)23.2 ± 0.49#&19.5 ± 0.57*#&35.1 ± 0.9737.5 ± 0.57