Abstract: FR-PO633

The Effects of Açai (Euterpe oleracea) Extract in the Oxidative Stress and Inflammation in Mouse Mesangial Cells Stimulated with High Glucose

Session Information

Category: Diabetes

  • 501 Diabetes Mellitus and Obesity: Basic - Experimental

Authors

  • Higa, Elisa Mieko Suemitsu, Medicine Department/Unifesp, Sao Paulo, Brazil
  • Lima, Deyse, UNIFESP, Sao Paulo, Brazil
  • Punaro, Giovana, UNIFESP, Sao Paulo, Brazil
  • Rodrigues, Adelson, UNIFESP, Sao Paulo, Brazil
  • Mouro, Margaret G, UNIFESP, Sao Paulo, Brazil

Group or Team Name

  • Laboratory of Nitric Oxide and Oxidative Stress
Background

Diabetes mellitus is a chronic disease characterized by hyperglycemia, which generates oxidative stress, with injuries to several organs. 20 to 30% of diabetic patients develop nephropathy, characterized by excessive production of extracellular mesangial matrix, marked initially by albuminuria, with gradual reduction of renal function. Açai is a native fruit from Amazon, which could provide beneficial effects on human health due to its antioxidant properties. The aim of this study was to evaluate the effects of açai extract (EA) in the oxidative stress and inflammation induced by high glucose in immortalized mouse mesangial cells (MiMC).

Methods

MiMC were cultured in DMEM with 5% fetal bovine serum. At 60-70% of confluence, they were cultured in media with normal glucose (NG – 6.7mmol/L), mannitol (osmolar control – 30mmol/L) or high glucose (30mmol/L) for 24, 48 or 72h. After the treatment, cell viability was assessed through an automated counter (CoutessTM, Invitrogen, USA). The supernatant was collected for measuring NO by Nitric Oxide Analyzer (NOATM, Sievers, CO, USA). NO was also measured in the cells by DAF-FM staining; reactive oxygen species (ROS) were measured using DCFH-DA. Catalase, Nrf2, iNOS and NF-κB p65 were analyzed by Western blot.

Results

The cell viability after treatment with EA remained greater than 90% in all groups, showing no cytotoxicity of the extract. There was a significant increase of the cellular proliferation in the HG when compared to the NG group, while EA managed to decrease it in all times studied. NO, ROS and inflammatory mediators were increased in the HG vs NG group, being also decreased after treatment with EA, p <0.05. HG showed a decrease in the protein content of the antioxidants catalase and Nrf2 vs NG group, which were partially recovered by EA, with p <0.05.

Conclusion

In this study, EA was able to decrease the proliferation and oxidative stress induced by high glucose, in MiMC, by suppressing inflammatory mediators signalling (NF-κB p65 and iNOS), and at the same time activating Nrf2/ antioxidant response pathways. The use of extract of açai could be an additional protective strategy for increase the antioxidant defense system in diabetes, and delay the progression of this disease and its complications, such as the nephropathy.

Funding

  • Government Support - Non-U.S.