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Kidney Week

Abstract: SA-PO559

The Quantitative and Qualitative Characteristics of Glomerular Basement Membrane in Rat Kidney Development and Glomerulonephritis

Session Information

  • Developmental Biology
    November 04, 2017 | Location: Hall H, Morial Convention Center
    Abstract Time: 10:00 AM - 10:00 AM

Category: Developmental Biology and Inherited Kidney Diseases

  • 401 Developmental Biology

Authors

  • Fei, Liang, China medical university, Shenyang, China
  • Kang, Dedong, Nippon Medical School, Tokyo, Japan
  • Lan, Ping, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
  • Nagasaka, Shinya, Nippon Medical School, Tokyo, Japan
  • Shimizu, Akira, Nippon Medical School, Tokyo, Japan
Background

The renal glomerulus is filtrate function of primary urine through glomerular tuft, which consists of podocytes, glomerular basement membrane (GBM) and endothelial cells. Mature GBM is mainly composed of α3/α4/α5 chain of type IV collagen (IV).

Methods

In order to clarify the maturation process of renal glomerular tuft during renal development, and injured and recovery of glomerular tuft from injuries in glomerulonephritis (GN), we evaluated the pathology and α chains (IV) of GBM and glomerular capillaries of 1-day-old and 10-week-old rat kidneys, as well as experimental Thy-1 GN on day 7 and 10 (n=3) and clinical biopsy cases of membranoprolferative GN (n=7).

Results

In the development, glomerular capillaries with endothelial cells migrated from interstitium into the cleft of S-shape body to form the glomerulus. The basement membrane (BM) of S-shape nephron and glomerular capillaries formed GBM, which were mainly composed by α1/α1/α2 chain (IV) in S-shape stage. In the early capillary loop stage of glomerulus, the cleft of the S-shaped body was occupied by a primitive capillary network. GBM was consisted of two BMs, such as continuous GBM of primitive podocytes with α3/α4/α5 chain (IV) and discontinuous capillary BM with α1/α1/α2 chain (IV). At late capillary loop stage of glomerulus, two layers of GBM such as continuous podocytes’BM and discontinuous or continuous capillary BM change to one layer of GBM with only α3/α4/α5 chain (IV). In early to late capillary stage, formation of foot processes and fenestrated structures was found in podocytes and endothelial cells, respectively. In experimental and biopsy cases of GN, injured glomerular tuft showed double contour of GBM, α3/α4/α5 chain (IV) of podocytes’GBM and α1/α1/α2 chain (IV) of capillary BM, with irregular effacement of foot processes of podocytes and irregular loss of fenestra of endothelial cells, resembling the pathology of glomerular tuft of early capillary stage. However, after recovery, glomerular tuft showed one layer of GBM with α3/α4/α5 chain (IV) and mature podocytes and endothelial cells.

Conclusion

Our findings suggest that injured glomerular tuft was accompanied by quantitative and qualitative alterations of GBM in GN, and recovery of glomerular tuft after injuries may be following similar processes in the development of glomerular tuft.