Abstract: TH-PO1090
Inflammatory Markers in Pediatric Nephrolithiasis
Session Information
- Mineral Disease: Nephrolithiasis
November 02, 2017 | Location: Hall H, Morial Convention Center
Abstract Time: 10:00 AM - 10:00 AM
Category: Mineral Disease
- 1204 Mineral Disease: Nephrolithiasis
Authors
- Kusumi, Kirsten, Akron Children''s Hospital, Akron, Ohio, United States
- Ketz, John, Nationwide Children''s Hospital, Columbus, Ohio, United States
- Schwaderer, Andrew L., Nationwide Children''s Hospital, Columbus, Ohio, United States
Background
Kidney stones associate with higher rates of heart attack/stroke and low bone mineral density/fractures. The mechanism of this association remains unknown; inflammation may be key. Our objective is to determine if pediatric stone formers have inflammation.
Methods
Urine was collected from 11 stone formers and 16 controls; 12-17 years old with radiographic evidence of stones. Exclusion criteria: metabolic acidosis, CKD II or >, immobilization, hyperlipidemia, hypertension, diabetes, inflammatory bowel disease, rheumatoid arthritis, or lupus. Urine was tested via a Mesoscale V-Plex Human Cytokine panel inflammation array. Levels were normalized to creatinine to control for concentration. Statistics by paired T-Test; p value <0.05 was significant.
Results
Five inflammatory markers were significantly increased in stone children: Il-13, Il-1B, MIP-1B, Il-12/Il-23p40 and Il-16.
Conclusion
This is the first evidence of inflammation in pediatric stone formers; this population is free of confounding diseases that are common in adults. Identification of inflammation in pediatric stone formers may be the first step in delineating the molecular mechanisms linking stone, bone and cardiovascular disease.
Table 1
| Inflammatory Marker | Control (pg/mg) | Stone former (pg/mg) | P value | |
| IL-12/IL-23P40 | 0.167 | 0.403 | 0.017 | |
| IFNY | 0 | 0 | 0.407 | |
| Il-10 | 0 | 0 | 0.999 | |
| Il-12p70 | 0 | 0 | 0.056 | |
| Il-13 | 0 | 0.08 | 0.003 | |
| Il-1B | 0.011 | 0.294 | 0.01 | |
| Il-2 | 0 | 0 | 0.937 | |
| Il-4 | 0 | 0 | 0.067 | |
| Il-6 | 0.255 | 0.366 | 0.394 | |
| Il-8 | 17.4 | 50.15 | 0.162 | |
| TNFA | 0 | 0 | 0.157 | |
| Eotaxin | 5.158 | 8.273 | 0.107 | |
| Eotaxin-3 | 1.045 | 0.886 | 0.514 | |
| IP-10 | 7.888 | 8.932 | 0.544 | |
| MCP-4 | 9.593 | 14.22 | 0.568 | |
| MCP-1 | 95.37 | 119.7 | 0.301 | |
| MCD | 1.519 | 1.957 | 0.438 | |
| MIP-1A | 0 | 0 | 0.371 | |
| MIP-1B | 2.582 | 5.829 | 0.0004 | |
| GM-CSF | 0.047 | 0.114 | 0.441 | |
| TARC | 0 | 0.004 | 0.396 | |
| Il-15 | 0.241 | 0.134 | 0.394 | |
| Il-16 | 0 | 0.2863 | 0.013 | |
| Il-17 | 0 | 0 | 0.183 | |
| Il-1A | 1.587 | 4.549 | 0.365 | |
| IL-7 | 0.49 | 0.506 | 0.862 | |
| TNFB | 0 | 0 | 0.688 | |
| VEGFA | 152.5 | 171.5 | 0.08 | |
| IL-5 | none detected | none detected | NA | |
MW: Mann–Whitney test