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Abstract: FR-PO229

Kidney Organoids Generated from Bone Marrow-Derived Mesenchymal Stem Cells

Session Information

Category: Cell Biology

  • 202 Apoptosis, Proliferation, Autophagy, Cell Senescence, Cell Transformation

Author

  • Chen, Xiangmei, Chinese PLA General Hospital, Beijing, BEIJING, China
Background

Via the directed differentiation of stem cells, progenitors can be induced to both collecting duct and nephrons. The human kidney originates from intermediate mesoderm. Cells cephalad migrate from the primitive streak (presomitic mesoderm) to form the intermediated mesoderm. The intermediate mesoderm increases the number of key renal progenitor cells, as well as the ureteral epithelium and the metanephric mesenchyme, which respectively form the collecting ducts and nephrons.
According to this theory, we have used bone marrow-derived mesenchymal stem cells (BM-MSCs) to differentiate into the kidney organoids by each other.

Methods

1) By simulating the regulation of CHIR99021-FGF9 cytokines, and controlling the ratio of the formation of the ureteral epithelium and the metanephric mesenchyme, the kidney organoids were constructed by MSCs. 2) The kidney specific markers in the kidney organoids were detected by immunofluorescence staining. 3)The electron microscope was used to observe the structure of the organoids.

Results

1) Using MSCs to differentiate into the kidney organoids through the regulation of cytokines. 2) Within these organoids, a single renal unit is divided into distal tubules, proximal tubules and glomeruli, which contains podocytes and blood vessels.

Conclusion

Such kidney organoids generated from MSCs represent powerful models of the human organ for future applications, including nephrotoxicity screening, disease modelling and as a source of cells for therapy.

Funding

  • Government Support - Non-U.S.