Kidney Week

Abstract: FR-PO629

Diabetes Aggravate Post-Ischemic Renal Fibrosis through Persistent Activation of Sonic Hedgehog Signaling

Session Information

• Diabetes Mellitus and Obesity: Basic - Experimental - II
  November 03, 2017 | Location: Hall H, Morial Convention Center
  Abstract Time: 10:00 AM - 10:00 AM

Category: Diabetes

• 501 Diabetes Mellitus and Obesity: Basic - Experimental

Authors

• Lee, Sang-Ho, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Seoul, Korea (the Republic of)

Sang-Ho Lee,

• Kim, Dong-Jin, KyungHee University, Seoul, Korea (the Republic of)

Dong-Jin Kim,

• Kang, Jun mo, Bundang CHA Medical Center, CHA Univ., Bundang, Korea (the Republic of)

Jun mo Kang,

• Park, Seon hwa, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Seoul, Korea (the Republic of)

Seon hwa Park,

• Song, Seok jong, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Seoul, Korea (the Republic of)

Seok jong Song,

• Kim, Su-Mi, Kyung Hee University , Seoul, Korea (the Republic of)

Su-Mi Kim,

• Jung-Woo, Seo, Kyung Hee University , Seoul, Korea (the Republic of)

Seo Jung-Woo,

• Lee, Yu ho, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Seoul, Korea (the Republic of)

Yu ho Lee,

• Kim, Yang gyun, Division of Nephrology Department of internal medicine Kyung Hee University College of Medicine, Seoul, Korea (the Republic of)

Yang gyun Kim,

• Moon, Ju young, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Seoul, Korea (the Republic of)

Ju young Moon,

• Lee, So-young, Bundang CHA Medical Center, CHA Univ., Bundang, Korea (the Republic of)

So-young Lee,

Background

Diabetes has a high risk for chronic kidney disease (CKD) and increases the severity of acute kidney injury (AKI). AKI induces renal fibrosis, known as a key feature of CKD, and renal fibrosis is associated with transforming growth factor-β1 (TGF-β1) and sonic hedgehog (Shh) signaling pathway. However, it is not known whether diabetes accelerates CKD progression via Shh signaling after AKI. Here, we investigated the influence of diabetes on CKD progression after AKI.

Methods

We established unilateral renal ischemia–reperfusion injury (IRI) model in streptozotocin induced diabetic mice. Histological changes in the kidney were evaluated at 3 and 5 weeks after IRI. The expression levels of mRNAs and proteins related to fibrosis and inflammation were determined by qRT-PCR and western blot. The effect of hyperglycemia on the epithelial-mesenchymal transition (EMT) induced by TGF-β1 and Shh signaling pathway was demonstrated in HKC-8.

Results

When comparing between 3 and 5 weeks after IRI, there was no improvement of tubulointerstitial injury in diabetes. Renal fibrosis was significantly higher in diabetes than in non-diabetes at 5 weeks after IRI. The pattern of infiltrated T and B cell was also consistent with that of renal fibrosis. The mRNA and protein expression levels of related TGF-β1 and Shh signaling pathway were significantly higher in the diabetic IRI kidneys than in the non-diabetic IRI kidneys. In vitro, Hyperglycemia led to the expression levels of TGF-β1 and Shh, and then interacted with each other for the progression of fibrosis.

Conclusion

Taken together, we demonstrated that diabetes on IRI induced the persistent activation of TGF-β1 and Shh signaling pathway and aggravate the interstitial renal fibrosis. In addition, the susceptibility to TGF-β₁ and Shh was increased by hyperglycemia in renal tubular cells.