Abstract: FR-PO1008

Everolimus Has Effect on the Tacrolimus Concentration within the Renal Proximal Tubular Cells in Dose Dependent Manners

Session Information

Category: Transplantation

  • 1701 Transplantation: Basic and Experimental

Authors

  • Lee, Haewon, Haeundae Paik Hospital, inje university, Pusan, Korea (the Republic of)
  • Kim, Yang Wook, Haeundae Paik Hospital, inje university, Pusan, Korea (the Republic of)
  • Park, Bongsoo, Haeundae Paik Hospital, inje university, Pusan, Korea (the Republic of)
  • Park, Sihyung, Haeundae Paik Hospital, inje university, Pusan, Korea (the Republic of)
  • Lee, Yoo jin, Haeundae Paik Hospital, inje university, Pusan, Korea (the Republic of)
  • Park, Seok ju, Inje University, Busan, Korea (the Republic of)
  • Han, Sang Youb, Inje University, Busan, Korea (the Republic of)
Background

It was reported that everolimus in combination with varying levels of Tacrolimus is efficacious and associated with good kidney function. We aimed to investigate the effect of everolimus on the tacrolimus intracelluar concentration in the kidney cells when it is used concomitantly.

Methods

HK-2 Cells(immortalized Human Renal Proximal Tubule Cells) were treated with tacrolimus at the dose of 5 ng/ml, 10 ng/ml and 15 ng/ml. After 30 minutes, we treated with everolimus at the dose of 3 ng/ml, 9 ng/ml and 15 ng/ml, additionally, in the absence or presence of tacrolimus for 1 hour. We measured the intracelluar tacrolimus concentrations using LC/MSMS.

Results

Tacrolimus intracellular accumulation was significantly decreased by everolimus in a concentration dependent manner. At the concomitant treatment with 5 ng/ml of tacrolimus and everolimus (3 ng/ml, 9 ng/ml, 15 ng/ml), the intracellular tacrolimus concentrations was significantly decreased compared with abscence of everolimus. The intracellular tacrololimus was decreased by everolimus showing 0.348± 0.025 ng/ml/1x106cells, 0.279± 0.034 ng/ml/1x106cells, 0.232± 0.025 ng/ml/1x106cells vs 0.643± 0.087 ng/ml/1x106cells (P=0.01), respectively. At the concomitant treatment with 10 ng/ml of tacrolimus and everolimus (3 ng/ml, 9 ng/ml, 15 ng/ml), the intracellular tacrolimus concentrations was significantly decreased compared with abscence of everolimus,The intracellular tacrololimus was decreased by everolimus showing 0.544± 0.103 ng/ml/1x106cells, 0.393± 0.042 ng/ml/1x106cells, 0.376± 0.06 ng/ml/1x106cells vs 0.71± 0.177 ng/ml/1x106cells,(P=0.042), respectively. The effect of everolimus on intracelluar tacrolimus concentration decreased as the dose of tacrolimus increased. At the concomitant treatment with 15 ng/ml of tacrolimus and everolimus (3 ng/ml, 9 ng/ml, 15 ng/ml), the intracellular tacrolimus concentrations was significantly decreased compared with abscence of everolimus. The intracellular tacrololimus was decreased by everolimus showing 0.654± 0.043 ng/ml/1x106cells, 0.635± 0.096 ng/ml/1x106cells,0.579± 0.251 ng/ml/1x106cells, vs 0.822± 0.168 ng/ml/1x106cells,(P=0.038), respectively.

Conclusion

We suggest that everolimus has effect on the tacrolimus concentration within the kidney proximal tubular cells in dose dependent manners.