Abstract: TH-PO429

In Vivo Responses of Phosphorus-Based Food Additives with Different Forms

Session Information

Category: Nutrition, Inflammation, and Metabolism

  • 1401 Nutrition, Inflammation, Metabolism

Authors

  • Fujii, Toru, Tokushima University, Tokushima, Japan
  • Kawabata, Yuka, Tokushima University, Tokushima, Japan
  • Segawa, Hiroko, Tokushima University, Tokushima, Japan
  • Hanazaki, Ai, Tokushima University, Tokushima, Japan
  • Ikuta, Kayo, Tokushima University, Tokushima, Japan
  • Kushi, Aoi, Tokushima University, Tokushima, Japan
  • Kaneko, Ichiro, Tokushima University, Tokushima, Japan
  • Tatsumi, Sawako, Tokushima University, Tokushima, Japan
  • Miyamoto, Ken-ichi, Tokushima University, Tokushima, Japan
Background

Hyperphosphatemia causes hyperparathyroidism and ectopic calcification in patients with chronic kidney disease, and dietary management for blood phosphate levels in patients with kidney disease is considered to be important. Both organic and inorganic phosphorus (Pi) are present in regularly consumed foods, such as eggs, and daily products. Pi is included in foods as an additive. Phosphorus-containing food additives were included with several forms (mono/polyphosphate-salt e.t.c.). In the small intestine, the luminal mono-phosphate can be available for absorption following ingestion of a food. Previous report suggested that polyphosphate salt have more harmful effects than those of monophosphate salt on bone physiology and renal function. The mechanism, however, has not been clarified. Recent studies suggested the presence of an gastro-renal signaling axis for dietary Pi as well as the existence of a mechanisms of intestinal Pi sensing, however, unknown. We focused that different forms of phosphorus-containing food additives have different effects in the body. In the present study, to clarify the mechanism, we investigated several responses of diet containing mono or polyphosphate on whole body.

Methods

C57B6 male mice were fed a test diet (low Pi diet, control Pi diet, high Pi diet 1 and 2) for short, middle and long periods. KH2PO4 (CP and HP1), and K5P3O10 (HP2) were used for phosphate additives.

Results

There were remarkable differences on blood, fecal, and urine biochemical analysis data between HP1 and HP2 diet group. Though HP1 and HP2 diet significantly increased inflammatory markers mRNA levels in several tissues, only HP2 diet increased fibrosis marker mRNA level in the kidney, urinary volume, and renal calcification. To identify the different response between HP1 and HP2 diet, renal and intestinal Pi regulating factors expression and activity were examined. There were no significantly differences on renal Pi regulating molecules between HP1 and HP2. However, we found differences on several intestinal molecules expression and activity levels between HP1 and HP2.

Conclusion

Intestine might detects difference luminal monophosphate and triphosphate form. It is necessary to consider about not only the phosphorus content but also the form of the phosphorus-containing food additives.

Funding

  • Government Support - Non-U.S.