Abstract: SA-PO764

Acute Effects of Hemodialysis on Circulating Microparticle Levels

Session Information

Category: Dialysis

  • 601 Standard Hemodialysis for ESRD

Authors

  • Xiao, Fengxia, Ottawa Hospital Research Institute, Ottawa, Alberta, Canada
  • Abujrad, Hussein, University of Ottawa, Ottawa, Ontario, Canada
  • Ooi, Teik Chye, University of Ottawa, Ottawa, Ontario, Canada
  • Sorisky, Alexander, Ottawa Hospital Research Institute, Ottawa, Alberta, Canada
  • Ruzicka, Marcel, None, Ottawa, Ontario, Canada
  • Burger, Dylan, Kidney Research Centre, Ottawa, Ontario, Canada
Background

Individuals with end stage kidney disease (ESKD) are at increased risk of cardiovascular complications. Previous studies have shown that levels of circulating microparticles are increased in ESKD and that an increase in circulating endothelial microparticles is a predictor of cardiovascular morbidity and mortality (Amabile, 2012). The purpose of this study was to examine the effect of a single hemodialysis session on levels of circulating microparticles in patients with ESKD.

Methods

We studied 23 patients (age 58±3 years, 12 M/11F) undergoing a single hemodialysis session. Levels of circulating total, endothelial, leukocyte, and platelet microparticles were assessed by flow cytometry immediately prior to, and at the completion of, hemodialysis (3 times weekly, 4 hour sessions).

Results

Participants had been treated by hemodialysis for 50±8 (mean±SEM) months prior to enrollment. The mean ultrafiltration volume on the day of study was 2.06 ±0.17 L. The level of total microparticles was significantly reduced by ~55% following hemodialysis (5.15x107±1.1 x107 [Pre] vs 2.32x107±4.27x106 [Post], P<0.05). Similarly, the level of platelet microparticles was significantly reduced by ~75% following hemodialysis (4.33x107±1.06 x107 [Pre] vs 1.06x107±3.8x106 [Post], P<0.05). In contrast, the level of leukocyte microparticles was not altered by hemodialysis (2.65x106±3.19 x105 [Pre] vs 2.15x106±3.2x105 [Post], P=n.s). The level of endothelial microparticles also remained the same before and after hemodialysis (2.09x106±4.85 x105 [Pre] vs 1.39x106±2.94x105 [Post], P=n.s). There was no correlation between the degree of ultrafiltration and the reduction in platelet, leukocyte, or endothelial microparticles.

Conclusion

Hemodialysis is associated with reductions in circulating total and platelet microparticles with no impact on circulating endothelial or leukocyte microparticles. These results suggest that dialytic clearance selectively influences the levels of circulating microparticle subpopulations in ESKD patients undergoing hemodialysis. Consideration should therefore be given to the timing of sampling for circulating microparticles in any study involving hemodialysis patients.

Funding

  • Government Support - Non-U.S.