Abstract: FR-PO801
Small Animal Study and Hemocompatibility of Small Form Factor Microfluidic Filtration System with Nitride Membranes
Session Information
- Standard Hemodialysis for ESRD - II
November 03, 2017 | Location: Hall H, Morial Convention Center
Abstract Time: 10:00 AM - 10:00 AM
Category: Dialysis
- 601 Standard Hemodialysis for ESRD
Author
- Johnson, Dean G., University of Rochester, Rochester, New York, United States
Background
Improving the health outcomes for End Stage Renal Disease (ESRD) patients on hemodialysis (HD) requires new technologies for wearable HD such as a highly efficient membrane that can achieve standard toxic clearance rates in small device footprints. Our group has developed nanoporous silicon nitride (NPN) membranes which are 100 to 1000 times thinner than conventional membranes and are orders-of-magnitude more efficient for dialysis.
Methods
Devices were constructed with polydimethylsiloxane (PDMS) and two NPN membrane chips. Uremic rats were dialyzed for 4 hours under anesthesia. The concentration of thrombin-anti-thrombin complex (TAT) and C3a complement was used as the indicator of coagulation and immune activation. For platelet adhesion and activation, platelet rich plasma (PRP) was incubated in microchannels then incubated with antibodies.
Results
The clearance results from the commercial membranes and the sham dialysis showed no reduction in urea concentration (Figure 1A). The NPN membranes from SiMPore, on the other hand reduced the urea by 26%. Albumin was retained during the 4-hour HD session (n=3) as expected. This indicates that the molecular weight cut-off was somewhere between the size of urea (60.06 Da) and that of albumin (66.5 kDa). The hemocompatibility results are shown in Figure 1B-D.
Conclusion
We had positive results from the NPN membranes clearing Urea better than commercial materials. Significantly, urea was cleared with very little membrane surface area. The favorable hemocompatibility results of the native silicon nitride are encouraging.
A: Sham dialysis, PES, and CTA membrane dialysis – no reduction in serum urea. 50 nm and 75 nm NPN membranes show a 24% decrease in serum urea. B: Coagulation (TAT) n = 3 C: Immune activation (C3a) n = 3 D: Platelet activation (CD62P) n = 15 for ADP, glass, and NPN; n = 5 for teflon.
Funding
- NIDDK Support