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Abstract: SA-PO187

AGE Content of a Protein Load Is Responsible for Renal Hemodynamical Modifications

Session Information

Category: Nutrition, Inflammation, and Metabolism

  • 1401 Nutrition, Inflammation, Metabolism

Authors

  • Normand, Gabrielle Laetitia, Hospices Civils de Lyon, Lyon----, France
  • Lemoine, Sandrine, Edouard Herriot Hospital, Lyon, France
  • Villien, Marjorie, CERMEP imagerie du vivant, Tassin la demi lune, France
  • Le bars, Didier, CERMEP, BRON, France
  • Merida, Ines, CERMEP, BRON, France
  • Irace, Zacharie, cermep, BRON, France
  • Troalen, Thomas, Siemens Healthcare S.A.S., Lyon, France
  • Costes, Nicolas, CERMEP - Imagerie du vivant, Lyon, France
  • Juillard, Laurent, University of Lyon, Lyon, France
Background

Low-protein diet is recommended to slow down chronic kidney disease progression because each protein load leads to a detrimental glomerular hyperfiltration. Protein preparations used to demonstrate protein-mediated renal hemodynamic effects were rich in AGE. The aim of our study was to evaluate if the AGE content of a protein load is responsible for protein-induced renal hemodynamic modifications.

Methods

Ten healthy subjects were assigned to a high-protein (1g/kg) low-AGE (3.000 kU AGE) versus high-AGE (30.000 kU AGE) meal, during imaging sessions performed on two different days. Renal perfusion assessed by PET using [15O] H2O, and renal oxidative metabolism measured by PET using [11C] labeled acetate, were measured before and 120- minutes after each meal.

Results

Renal perfusion increased significantly (3.16 ± 0.55 to 3.80 ± 0.42 mL/min/g, p=0.0002) after the high-AGE meal whereas it was not modified after the low-AGE meal (3.35±0.65 to 3.38±0.53 ml/min/g, p=0.88) (Table 1 and Figure 1). Oxidative metabolism increased significantly after the high-AGE meal (0.3 ± 0.04 vs 0.36 ± 0.08 min-1, p=0.005) compared to the low-AGE meal (0.30 ± 0.02 vs 0.31 ± 0.06 min-1, p=0.76) for both cortices.

Conclusion

This is not the high protein content of a meal that increases renal perfusion and oxidative metabolism but its high-AGE content. This study suggests that prevention of CKD progression should aim predominantly at reducing food AGE content.

Renal functional parameters.
 Low-AGE (n = 9)High-AGE (n = 10)
AcquisitionsBaselinePost prandialBaselinePost prandial
15O-water PET (ml/g/min)-3.35 ± 0.653.38 ± 0.533.16 ± 0.553.8 ± 0.42 *
11C- acetate PET (min-1)-0.30 ± 0.020.31 ± 0.060.30 ± 0.040.36 ± 0.08 *
BOLD-MRI (Cortical R2*)18.3 ± 1.320.4 ± 2.7 *17.9 ± 1.220.1 ± 3.3
BOLD-MRI (medullary R2*)27.6 ± 3.232.2 ± 4.1*27.1 ± 4.932.4 ± 5.7 *

* means p < 0.05