Abstract: SA-PO187
AGE Content of a Protein Load Is Responsible for Renal Hemodynamical Modifications
Session Information
- Nutrition, Inflammation, Metabolism: Clinical Trials, Biomarkers, Epidemiology
November 04, 2017 | Location: Hall H, Morial Convention Center
Abstract Time: 10:00 AM - 10:00 AM
Category: Nutrition, Inflammation, and Metabolism
- 1401 Nutrition, Inflammation, Metabolism
Authors
- Normand, Gabrielle Laetitia, Hospices Civils de Lyon, Lyon----, France
- Lemoine, Sandrine, Edouard Herriot Hospital, Lyon, France
- Villien, Marjorie, CERMEP imagerie du vivant, Tassin la demi lune, France
- Le bars, Didier, CERMEP, BRON, France
- Merida, Ines, CERMEP, BRON, France
- Irace, Zacharie, cermep, BRON, France
- Troalen, Thomas, Siemens Healthcare S.A.S., Lyon, France
- Costes, Nicolas, CERMEP - Imagerie du vivant, Lyon, France
- Juillard, Laurent, University of Lyon, Lyon, France
Background
Low-protein diet is recommended to slow down chronic kidney disease progression because each protein load leads to a detrimental glomerular hyperfiltration. Protein preparations used to demonstrate protein-mediated renal hemodynamic effects were rich in AGE. The aim of our study was to evaluate if the AGE content of a protein load is responsible for protein-induced renal hemodynamic modifications.
Methods
Ten healthy subjects were assigned to a high-protein (1g/kg) low-AGE (3.000 kU AGE) versus high-AGE (30.000 kU AGE) meal, during imaging sessions performed on two different days. Renal perfusion assessed by PET using [15O] H2O, and renal oxidative metabolism measured by PET using [11C] labeled acetate, were measured before and 120- minutes after each meal.
Results
Renal perfusion increased significantly (3.16 ± 0.55 to 3.80 ± 0.42 mL/min/g, p=0.0002) after the high-AGE meal whereas it was not modified after the low-AGE meal (3.35±0.65 to 3.38±0.53 ml/min/g, p=0.88) (Table 1 and Figure 1). Oxidative metabolism increased significantly after the high-AGE meal (0.3 ± 0.04 vs 0.36 ± 0.08 min-1, p=0.005) compared to the low-AGE meal (0.30 ± 0.02 vs 0.31 ± 0.06 min-1, p=0.76) for both cortices.
Conclusion
This is not the high protein content of a meal that increases renal perfusion and oxidative metabolism but its high-AGE content. This study suggests that prevention of CKD progression should aim predominantly at reducing food AGE content.
Renal functional parameters.
| Low-AGE (n = 9) | High-AGE (n = 10) | |||
| Acquisitions | Baseline | Post prandial | Baseline | Post prandial |
| 15O-water PET (ml/g/min)- | 3.35 ± 0.65 | 3.38 ± 0.53 | 3.16 ± 0.55 | 3.8 ± 0.42 * |
| 11C- acetate PET (min-1)- | 0.30 ± 0.02 | 0.31 ± 0.06 | 0.30 ± 0.04 | 0.36 ± 0.08 * |
| BOLD-MRI (Cortical R2*) | 18.3 ± 1.3 | 20.4 ± 2.7 * | 17.9 ± 1.2 | 20.1 ± 3.3 |
| BOLD-MRI (medullary R2*) | 27.6 ± 3.2 | 32.2 ± 4.1* | 27.1 ± 4.9 | 32.4 ± 5.7 * |
* means p < 0.05