Abstract: SA-PO858

Combined Therapy of Menaquinone-7 and Omega-3 Fatty Acid Prevents Progression of Aortic Calcification in Adenine and Low Protein Diet Induced Rat Model

Session Information

  • Vascular Calcification
    November 04, 2017 | Location: Hall H, Morial Convention Center
    Abstract Time: 10:00 AM - 10:00 AM

Category: Mineral Disease

  • 1205 Vascular Calcification

Authors

  • Lee, Su mi, Dong-A University, Busan, Not Applicable, Korea (the Republic of)
  • Choi, Hyuck jae, Dong-A University, Busan, Not Applicable, Korea (the Republic of)
  • Kim, Kitae, BHS Han Seo Hospital, Busan, Korea (the Republic of)
  • Son, Sung Hyun, BHS Han Seo Hospital, Busan, Korea (the Republic of)
  • Son, Young ki, Dong-A University, Busan, Not Applicable, Korea (the Republic of)
  • Kim, Seong Eun, Dong-A University, Busan, Not Applicable, Korea (the Republic of)
  • An, Won Suk, Dong-A University, Busan, Not Applicable, Korea (the Republic of)
Background

Vascular calcification is common and progressing in chronic kidney disease and dialysis patients. Diet with high-dose menaquinone-7 (MK-7) (100 ug/g diet) inhibited the development of cardiovascular calcification in 5/6 nephrectomy rat combined with high phosphate diet. Eicosapentaenoic acid (1g/kg/day), one of omega-3 fatty acid (FA), attenuates arterial medial calcification induced by warfarin. We evaluated whether the effect of omega-3 FA and MK-7 on aortic calcification in adenine and low protein diet induced vascular calcification rat model.

Methods

Male Sprague Dawley rats were fed the diets containing 0.75% adenine and 2.5% protein for 3 weeks. After 3 weeks, 4 rats were sacrificed for calcification evaluation of thoracic aorta. Thirty two rats were randomly divided into four groups, which were treated and fed the diets containing 2.5% protein for 4 weeks: adenine control (0.9% saline), adenine control treated with omega-3 FA (300 mg/kg/day by gastric gavage), adenine control treated with MK-7 (50 ug/kg/day by gastric gavage), adenine control treated with omega-3 FA and MK-7. Normal control rats were fed the diets containing 2.5% protein for 7 weeks. For quantitative assessment of aortic calcification, von Kossa stain of aorta was done and calcium contents were measured with calcium colorimetric kit.

Results

Serum creatinine of adenine control group treated with omega-3 FA and MK-7 was lower than adenine control group without treatment. Serum calcium and BUN levels were not significantly different between adenine control group with treatment and without treatment. Two rats among 4 rats showed aortic calcification at 3 weeks. After 4 weeks, aortic calcification was progressed in adenine control group without treatment on von Kossa stain and calcium contents analysis of aorta. Aortic calcification on von Kossa stain and calcium contents was the least progressed in adenine control group treated with combination of omega-3 FA and MK-7 compared to omega-3 FA or MK-7 single therapy.

Conclusion

Combined treatment with omega-3 FA and MK-7 definitely prevents progression of aortic calcification compared to rat without treatment in adenine and low protein diet induced vascular calcification rat model.