Abstract: TH-PO372

Resveratrol Attenuates Epithelial to Mesenchymal Transition of Human Renal Proximal Tubular Epithelial Cells by Induction of M2 Macrophages Polarization with High Expression of HO-1

Session Information

Category: Cell Biology

  • 201 Cell Signaling, Oxidative Stress

Authors

  • Li, Jun, Jiangnan University, Wuxi, China
  • Yu, Ya-Fen, Affiliated hospital of Jiangnan University, Wuxi, China
  • Zhang, Ting, Affiliated hospital of Jiang nan university, Wu xi, Jiangsu, China
Background

The aim of this study was to investigate the effect and mechanism of M2 macrophages with high expression of HO-1, which were induced with resveratrol, on TGF-β1 induced chronic tubular injury.

Methods

THP-1 cells (human leukemic monocyte) were treated with resveratrol concentration. HK-2 cells (human renal tubular epithelial cells) were treated with TGF-β1 to induce the chronic tubular damage. Co-culture technique was used to clarify the role of HO-1 positive M2 macrophages, which were induced by resveratrol, in the amelioration of chronic tubular injury.

Results

1.THP-1 cells were treated with resveratrol, ICC showed the positive staining of HO-1 and the markers of M2 macrophage(CD206 and Macrophage mannose receptor 2, Mrc-2), and the supernatant showed increased IL-10 levels and decreased IL-12 levels. The western blot of the THP-1 cells protein showed the increased expression of p-STAT3 and IL-10.
2. After co-cultured of injured HK-2 cells with THP-1 cells intervened by resveratrol, the ratio of G2/M phase was lower than that treated with TGF-β1 alone. The ICC of HK-2 cells showed the increased expression of E-cadherin and the decreased expression of α-smooth muscle actin(α-SMA). The western blot of the HK-2 cells protein also showed the decreased p-STAT3 expression.

Conclusion

Small doses of resveratrol can induce the expression of HO-1 and M2 macrophages polarization, which might help to attenuate the progression of fibrosis in HK-2 cells by STAT3 signaling pathway.

Figure 1 Co-cultured of TGF-β1 damaged HK-2 cells with THP-1 cells treated with resveratrol(20μmol/l)
A B. CD206, HO-1 of THP-1 cells; C D.α-SMA of injured HK-2 without/with co- culture with THP-1; E F. E-cadherin of injured HK-2 without/with co- culture with THP-1;

Funding

  • Government Support - Non-U.S.