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Abstract: TH-PO372

Resveratrol Attenuates Epithelial to Mesenchymal Transition of Human Renal Proximal Tubular Epithelial Cells by Induction of M2 Macrophages Polarization with High Expression of HO-1

Session Information

Category: Cell Biology

  • 201 Cell Signaling, Oxidative Stress


  • Li, Jun, Jiangnan University, Wuxi, China
  • Yu, Ya-Fen, Affiliated hospital of Jiangnan University, Wuxi, China
  • Zhang, Ting, Affiliated hospital of Jiang nan university, Wu xi, Jiangsu, China

The aim of this study was to investigate the effect and mechanism of M2 macrophages with high expression of HO-1, which were induced with resveratrol, on TGF-β1 induced chronic tubular injury.


THP-1 cells (human leukemic monocyte) were treated with resveratrol concentration. HK-2 cells (human renal tubular epithelial cells) were treated with TGF-β1 to induce the chronic tubular damage. Co-culture technique was used to clarify the role of HO-1 positive M2 macrophages, which were induced by resveratrol, in the amelioration of chronic tubular injury.


1.THP-1 cells were treated with resveratrol, ICC showed the positive staining of HO-1 and the markers of M2 macrophage(CD206 and Macrophage mannose receptor 2, Mrc-2), and the supernatant showed increased IL-10 levels and decreased IL-12 levels. The western blot of the THP-1 cells protein showed the increased expression of p-STAT3 and IL-10.
2. After co-cultured of injured HK-2 cells with THP-1 cells intervened by resveratrol, the ratio of G2/M phase was lower than that treated with TGF-β1 alone. The ICC of HK-2 cells showed the increased expression of E-cadherin and the decreased expression of α-smooth muscle actin(α-SMA). The western blot of the HK-2 cells protein also showed the decreased p-STAT3 expression.


Small doses of resveratrol can induce the expression of HO-1 and M2 macrophages polarization, which might help to attenuate the progression of fibrosis in HK-2 cells by STAT3 signaling pathway.

Figure 1 Co-cultured of TGF-β1 damaged HK-2 cells with THP-1 cells treated with resveratrol(20μmol/l)
A B. CD206, HO-1 of THP-1 cells; C D.α-SMA of injured HK-2 without/with co- culture with THP-1; E F. E-cadherin of injured HK-2 without/with co- culture with THP-1;


  • Government Support - Non-U.S.