Abstract: TH-PO488

Extracellular Vesicles (EV) Plus Swimming Exercise Training (EXE) Improve Creatinine Clearance (CrCl), Proteinuria, and Glomerulosclerosis and Decrease Mortality in Rats with CKD

Session Information

Category: Chronic Kidney Disease (Non-Dialysis)

  • 303 CKD: Epidemiology, Outcomes - Cardiovascular

Authors

  • Luiz, Rafael, Universidade Federal de São Paulo, São Paulo, Brazil
  • Rampaso, Rodolfo Rosseto, Universidade Federal de São Paulo, São Paulo, Brazil
  • Pessoa, Edson Andrade, Universidade Federal de São Paulo, São Paulo, Brazil
  • Gloria, Maria A., Universidade Federal de São Paulo, São Paulo, Brazil
  • Jorge, Luciana, Universidade Federal de São Paulo, São Paulo, Brazil
  • Silva, Kleiton Augusto Santos, University of Missouri, Columbia, Alabama, United States
  • Cesaretti, Mario luis Ribeiro, Universidade Federal de São Paulo, São Paulo, Brazil
  • Schor, Nestor, Universidade Federal de São Paulo, São Paulo, Brazil
Background

The aim of this study was to evaluate the EV and EXE on renal function and glomerulosclerosis in rats with by 5/6 Nephrectomy (5/6Nx).

Methods

Adult Wistar rats were divided in groups (n=8): Control (C), Exercise (E), Sedentary 5/6Nx (NS), Exercise 5/6Nx (NE), NS + EV (VSD) and NE + EV (VEX). The protocol was employed in 5/6Nx rats after 7 days from the surgical procedures. EXE periods were 60min/day, 5 days a week during 8 weeks and EV (100 μg) was induced into the tail vein. It was evaluated creatinine clearance (CrCl), proteinuria (uProt), glomerulosclerosis (%), mortality rate as well mean arterial pressure (MAP) and maximal exercise test (MEtest).

Results

The EV and EXE improve the CrCl vs NS group 0.96±0.20 ml/min, (p<0.05). Proteinuria was significantly different in VSE and VEX vs NE and NS groups. Glomerulosclerosis was higher in NS vs VSE and VEX (p<0.05). A higher mortality rate was observed in NS and VSE, but not in NE and VEX groups. BUN was normalized in NE, VSE and VEX vs NS. There is an increment in MAP but prevent, at least in part, a lower decline in the MEtest caused by 5/6Nx.

Conclusion

Results suggested that EV plus 8 weeks of EXE minimize the impact of 5/6Nx by decreasing of proteinuria, glomerulosclerosis and reduce the impact on CrCl. Finally, the decreasing mortality rate in NE and VEX vs NS and VSE indicate that exercise, at least swimming in this protocol, induced protection on renal function. Thus, it is reasonable to suggest that EV plus EXE could be an additional strategy to be employed in Chronic Kidney Disease.

 CENSNEVSEVEX
MAP (mmHg)125±1128±2220±16*#210±6*#
231±20*#212±11*#
MEtest (m/min)26±236±1*16±2"29±1#26±1#
36±1*@
CrCL/ (ml/min/BW)1.8±0.21.5±0.20.9±0.2*#2.3±0.3&1.1±0.1$1.2±0.0$
BUN (mg/dL)46.67±5.1044.90±5.18180.60±49.22"43.62±7.3057.8±10.851.1±4.2
uProt (mg/24h)12.00±1.1218.92±1.7440.13±2.35*#37.78±3.12*#30.2±3.5%30.9±2.6%
Glomerulosclerosis (%)005.8±22.8±0.61.5±0.31.2±2.2
Mortality Rate (%)0070393912

* vs C; # vs E; & vs NxS; @ vs C, NS, NE and VSE; % vs NS and NE; " vs