Abstract: SA-PO347

Omega 3 Fatty Acid Attenuates Kidney Fibrosis in Ureteral Obstructed Mice via Enhancement of Autophagy Flux

Session Information

Category: Chronic Kidney Disease (Non-Dialysis)

  • 308 CKD: Mechanisms of Tubulointerstitial Fibrosis

Authors

  • Choi, Dae Eun, Chungnam National University, Daejeon, Korea (the Republic of)
  • Chang, Yoon-Kyung, The Catholic University of Korea, Daejeon, Korea (the Republic of)
  • Choi, Hyunsu, Daejeon St. Mary's Hospital, Daejeon, Korea (the Republic of)
  • Song, Chang hun, Chungnam National University, Daejeon, Korea (the Republic of)
  • Lee, Jiwon M., Chungnam National University , Daejeon, Korea (the Republic of)
  • Na, Kiryang, Chungnam National University, Daejeon, Korea (the Republic of)
  • Lee, Kang Wook, Chungnam National University, Daejeon, Korea (the Republic of)
  • Bae, Hong jin, Chungnam National University, Daejeon, Korea (the Republic of)
  • Ham, Youngrok, Chungnam National University, Daejeon, Korea (the Republic of)
Background

It has been known that unilateral ureteral obstruction (UUO) induces autophagic activation in obstructed kidney. Inhibition of autophagy aggravates renal injury in UUO mice. Recently, it is reported that Omega 3 fatty acid regulate the autophagy. we evaluated whether ω3-PUFA may attenuate renal fbrosis in UUO mice, and evaluated associating mechanism.

Methods

10-week-old male C57Bl/6 mice were divided into 4 groups; sham, Omega 3 + sham, vehicle (normal saline, same volume to Omega 3 + UUO, Omega 3 + UUO. Omega 3 and vehicle were administered orally using an NG tube (Omega 3 100mg/kg/day) from pre-operation day to 7 days after operation. Mice were sacrifced at 7 days after surgery and kidney tissue were collected. Real time RT-PCR, western blot and immunohistochemistry for molecular study and H&E stain and PAS stain for histologic examination were performed.

Results

Omega 3 treated UUO mice showed improvement of renal cell survival, renal function, and pathologic damage compared to vehicle treated UUO mice. Also omega-3 treatment reduced the renal expression of MCP-1, collagen IV, and TGF-ß in UUO kidney. UUO mice kidney showed that higher amounts of LC3, Beclin-1, Atg7 and p62 compared to sham mice. Omega 3 treated UUO kidney showed higher amounts of LC3, Beclin-1 and Atg7 and lower amounts of p62 compared to vehicle treated UUO kidney. Moreover, renal cathepsin D and ATP6E were also increased in Omega 3 treated UUO mice compared to vehicle treated UUO mice mice.

Conclusion

Omega 3 fatty acid ameliorate renal fbrosis in UUO kidney via enhancement of autophagy fux.

Funding

  • Government Support - Non-U.S.