Abstract: SA-PO541

Study on Morphogenesis of Renal Vasculature Architecture Based on Computer-Assisted 3D Reconstruction

Session Information

  • Developmental Biology
    November 04, 2017 | Location: Hall H, Morial Convention Center
    Abstract Time: 10:00 AM - 10:00 AM

Category: Developmental Biology and Inherited Kidney Diseases

  • 401 Developmental Biology

Authors

  • Zhang, Jie, China Medical University, Shenyang, Liaoning, China
  • Yang, Jie, China Medical University, Shenyang, Liaoning, China
  • Chang, Shi-Jie, China Medical University, Shenyang, Liaoning, China
  • Gu, Ling, China Medical University, Shenyang, Liaoning, China
  • Wang, Kaiyue, China Medical University, Shenyang, Liaoning, China
  • Thomsen, Jesper Skovhus, Aarhus University, Aarhus, Denmark
  • Andreasen, Arne A, Aarhus University, Aarhus, Denmark
  • Christensen, Erik I., Aarhus University, Aarhus, Denmark
  • Zhai, Xiao-Yue, China Medical University, Shenyang, Liaoning, China
Background

The architecture of the renal vasculature (RV) is formed so that the renal function is highly efficient. However, a relationship has been suggested between the morphogenesis of RV and the progression of renal fibrosis in CKD, due to the close proximity of peritubular capillaries and tubules. Present study demonstrates the transformation of RV trees from young to adult kidneys based on histological serial sections, and provides a quantitative reference with establishment of microcirculation, aiming at better understanding the morphogenic processes, meanwhile the progression of the nephron pathophysiology.

Methods

Serial paraffin and epoxy sections from E14.5, E17.5, P5, and adult kidneys were prepared for vessel tracing on aligned micrographs, using custom-made computer software. In addition, glomerular and medullary capillaries volume densities were measured on sections immunostained for CD34, from 3 mouse kidneys of each of 11 developing days.

Results

At E14.5, the RV trees consisted of six main arteries running between cortex and medulla and dozens of lateral branches along them. These smaller branches further divide in even smaller branches connecting to the earliest formed immature glomeruli. Soon after, with rapidly increasing number of nephrons, a burst branching network of RV formed with tremendous lateral and bifid branching until P5. After that, with expansion of especially the medullary volume, the RV tree remodeled into the typical spatial arrangement, including main, arcuate, and interlobular arteries, as well as afferent and efferent arterioles (AA and EA). The typical medullary vascular bundles (VB) originated from the EAs of earlier born nephrons intimately accompanying with Henle’s ascending limb, and gradually formed by branching of the EA accompanied by more thin limbs and vessels when penetrating into the deep medulla. Consistently, the volume density of medullary microcirculation increased with the VB formed.

Conclusion

A large number of RV branches are completed by cessation of nephrogenesis. The RV is not only formed by iterative branching, but also by lateral and sprouting branches. In addition, the RV trees undergo remodeling corresponding to an increasing volume density, driven by the spatial arrangement of the neighboring tubules during the postnatal life.

Funding

  • Government Support - Non-U.S.