Abstract: TH-PO247
Omega-3 Fatty Acids Attenuate Cisplatin Nephrotoxicity via Enhancement of Autophagy Flux
Session Information
- AKI Basic: Cell Death and Biomarkers
November 02, 2017 | Location: Hall H, Morial Convention Center
Abstract Time: 10:00 AM - 10:00 AM
Category: Acute Kidney Injury
- 001 AKI: Basic
Authors
- Ham, Youngrok, Chungnam National University , Daejeon, Korea (the Republic of)
- Jeong, Jin young, Chungnam National University, Daejeon, Korea (the Republic of)
- Bae, Hong jin, Chungnam National University , Daejeon, Korea (the Republic of)
- Song, Chang hun, Chungnam National University , Daejeon, Korea (the Republic of)
- Na, Kiryang, Chungnam national university, Deajeon, Korea (the Republic of)
- Lee, Kang Wook, Chungnam National University , Daejeon, Korea (the Republic of)
- Kim, Jwajin, Chungnam national university, Deajeon, Korea (the Republic of)
- Lee, Jiwon M., Chungnam National University, Daejeon, Korea (the Republic of)
- Choi, Dae Eun, Chungnam National University , Daejeon, Korea (the Republic of)
Background
Various studies demonstrated that omega-3 polyunsaturated fatty acids (PUFAs) attenuate kidney injuries through anti-apoptotic and antioxidant properties. Recently, several studies showed that omega-3 PUFAs enhance induction of autophagy. We evaluated whether administration ω-3 PUFAs may induce autophagy in cisplatin nephrotoxicity, and investigated the role of autophagy in attenuating cisplatin nephrotoxicity by ω-3 PUFAs.
Methods
10-week- old male C57BL/6 mice were divided into 4 groups; control, control plus omega 3, cisplatin, cisplatin plus omega 3; they were injected with a vehicle or single dose of cisplatin (16 mg/kg body weight) intraperitoneally. Omega 3 and vehicle were administered orally using an NG tube (Omega 32,000 μg/kg/day) from pre-injection day to 3 days after injection of cisplatin. Mice were sacrificed at 4 days after administration of cisplatin and kidney tissue were collected. Real time PCR, western blot and immunohistochemistry for molecular study and H&E stain and PAS stain for histologic examination were performed.
Results
Omega 3 treated cisplatin-mice showed improvement of renal cell survival, renal function,and pathologic damage compared to vehicle treated cisplatin-mice. Omega-3 treatment also reduced the renal expression of MCP-1 (Monocytye chemotactic protein-1) and OPN (Osteopontin) in cisplatin-treated kidney. Cisplatin-mice kidney showed that higher amounts of LC3, Beclin-1 and p62 compared to sham mice. Omega 3 treated cisplatin kidney showed higher amounts of LC3 and Beclin-1 and lower amounts of p62 compared to vehicle treated cisplatin kidney. Moreover, renal cathepsin D and ATP6E were also increased in omega 3 treated cisplatin-mice compared to vehicle treated cisplatin-mice.
Conclusion
Omega 3 fatty acids attenuate renal injury in cisplatin nephrotoxicity through stimulating autophagy flux