Abstract: TH-PO315

Youthful Systemic Milieu Alleviates Renal Ischemia Reperfusion Injury in Elderly Mice

Session Information

Category: Acute Kidney Injury

  • 001 AKI: Basic

Author

  • Chen, Xiangmei, Chinese PLA General Hospital, Beijing, Beijing, China
Background

The incidence of acute kidney injury (AKI) is high in elderly people. Parabiosis is an experimental model that surgically joins the muscle and hypoderm of two organisms to develop a shared circulatory system. Within this common blood circulation, blood cells and soluble factors can exchange continuously at physiological levels. Parabiosis has been used to study the different internal environmental factors that affect organ function and recovery from damage. A young systemic environment may prevent the senescence of old organs.

Methods

The mice were divided into four groups at random.1). old sham group (O:sham), 2). old IRI group (O:IRI). 3). old-old parabiosis IRI group (O-O:IRI). 4). young-old parabiosis IRI group (Y-O:IRI). The parabiosis model was established first. Then 3 weeks, the bilateral renal pedicles were clamped in the old recipient mouse.

Results

It was verificated that cross-circulation in the parabiosis model with the IVIS Spectrum Imaging System, fluorescence microscopy, flow cytometry analysis. At 24 hours after IRI, compared to old wild-type mice, the old IRI mice had significantly damaged renal histology, decreased renal function, and increased renal tissue apoptosis. Compared to old IRI mice, old-old parabiosis IRI mice did not show differences in renal histological damage, renal tissue apoptosis. Compared to the old-old parabiosis IRI mice, the old IRI mice in the young-old parabiosis showed less renal histological injury and better renal function. In addition, the renal tissue expression levels of proteins related to apoptosis were significantly decreased.

Conclusion

These results indicate that a young systemic milieu may ameliorate renal ischemia reperfusion injuries in old mice.

Funding

  • Government Support - Non-U.S.