Abstract: SA-PO336

Previous Resistance Training Impact in CKD Leading to Improvements in Proteinuria, Creatinine Clearance, and Mortality Rate Reduction in Rats

Session Information

Category: Chronic Kidney Disease (Non-Dialysis)

  • 308 CKD: Mechanisms of Tubulointerstitial Fibrosis

Authors

  • Saud, Alexandre, UNIFESP, São Paulo, Brazil
  • Luiz, Rafael, UNIFESP, São Paulo, Brazil
  • Reinecke, Natalia, UNIFESP, São Paulo, Brazil
  • Silva, Wesley, Universidade Federal de São Paulo, Sao Paulo [SP], Brazil
  • Filho, Samuel Trindade, Universidade Federal de São Paulo, Sao Paulo [SP], Brazil
  • Rampaso, Rodolfo Rosseto, None, São Paulo, São PAULO, Brazil
  • Schor, Nestor, Universidade Federal de Sao Paulo/Escola Paulista de Medicina, Sao Paulo, Brazil
Background

The resistance training is applied to improve and increase muscle mass, however, how resistance exercise improves renal function is not fully understood.The aim of this study was to evaluate the EXE effects on renal function and mortality rate in rats with 5/6Nx

Methods

Adult Wistar rats were divided in four groups (n=8): Previous Exercise + Nx 5/6 + post surgery Exercise (ENE), Previous Exercise + Nx 5/6 + post surgery Sedentary (ENS), Sedentary + Nx 5/6 + post surgery Exercise (SNE) and Sedentary + Nx5/6 + post surgery Sedentary (SNS). We evaluated mean arterial pressure (MAP), creatinine clearance (CrCl), proteinuria (uProt), blood urea nitrogen (BUN), maximal load test (MLT), well as mortality rate. EXE was performed as follows: 6 to 12 climbs/day, 5 days a week, during 8 weeks, 40 to 60% of maximal load test

Results

Exercise in ENE group prevented the increase in proteinuria rate (45,7±3,6 mg/24h, p<0.05) vs SNS, Creatinine Clearance(1,3±0,1ml/min p<0,05) vs all and mean BUN(33,3±2,7 mg/dL p<0,05) was lower compared with the SNS and ENS groups. A gain of strength was observed in ENE vs all (556±35 g p<0,05). A lower mortality rate was observed in ENE(0%) vs SNS(62%). Results suggested that the EXE minimize the impact of 5/6Nx, with lower proteinuria(35%), Clearance of creatinine(40%), lower increase in BUN(35%) and gain of strength(30%)

Conclusion

Results suggested that the EXE minimize the impact of 5/6Nx, with lower proteinuria (35%), Clearance of creatinine (40%), lower increase in BUN (35%) and gain of strength (30%). These parameters indicate that exercise could have a protective effect, especially under this experimental protocol. Thus, this study suggests that the exercise plays a preventive role in mortality and could be an additional strategy to be employed in CKD.

 SNSSNEENSENE
Weight (g)337±13345±15370±7369±15
MLT (g)390±22411±33390±34556±35$
uProt (mg/24hrs)133±14$66.8±1387.3±7.445.7±3.6&
CrCl (ml/min)0.5±0.10.7±0.10.7±0.11.3±0.1$
BUN (mg/dL)52.3±543.1±450.1±3.533.3±2.7* &
Mortality Rate (%)62%11%27%0$
MAP (mmHg)216±6#176±13217±4.7#207±8.2

$ vs all; * vs SNS, # vs. SNE, & vs ENS.

Funding

  • Government Support - Non-U.S.