Abstract: FR-PO273

The Effect and Possible Mechanism of Klotho on the Expression of Fibroblastic Growth Factor 23(FGF23) Secreted by Osteoblast-Like UMR-106 Cells

Session Information

Category: Mineral Disease

  • 1202 Mineral Disease: Vitamin D, PTH, FGF-23

Authors

  • Mao, Huijuan, First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Ma, Lulu, First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Background

To investigate the effect and possible mechanism of Klotho on the expression of FGF23 in UMR- 106 cells.

Methods

UMR-106 cells were divided into 5 groups and cultured for 72 h: (1)control group;(2)β-glycerophosphate(β-GP) group;(3)β-GP+Klotho group;(4)β-GP+LiCl group;(5)β-GP+Klotho+LiCl group,and then the expression of FGF23, P-GSK-3β and GSK-3β protein was measured by Western blotting.The levels of mRNA of FGF23 and c-myc were determined by RT-PCR.

Results

1.β-GP induced the increase of expression of FGF23 mRNA and protein.Compared with β-GP group, FGF23 mRNA and protein expression was downregulated after treating with Klotho(Figure 1).2.β-GP induced the increase of expression of P-GSK-3β/GSK-3β and c-myc mRNA.Compared with β-GP group, P-GSK-3β/GSK -3β and c-myc mRNA were downregulated after treating with Klotho(Figure 2).3.The expression of FGF23, P-GSK-3β/GSK-3β and c-myc mRNA were upregulated when treated with LiCl(Figure 1 and 2).

Conclusion

Klotho down-regulates the expression of FGF23 induced by hyperphosphate in osteoblast-like UMR-106 cells via Wnt/β-catenin pathway.

Figure1:Effect of Klotho on the expression of FGF23 in UMR-106 cells.

Figure2:Klotho regulated the expression of FGF23 through Wnt/β-catenin signaling pathway.

Funding

  • Government Support - Non-U.S.