Abstract: TH-PO602
Effects of Moderate and High Intensity Chronic Exercise on Renal Function in PCK Rats of Polycystic Kidney Disease
Session Information
- Cystic Kidney Diseases - I
November 02, 2017 | Location: Hall H, Morial Convention Center
Abstract Time: 10:00 AM - 10:00 AM
Category: Genetic Diseases of the Kidney
- 801 Cystic Kidney Diseases
Authors
- Qiu, Jiahe, Tohoku University Graduate School of Medicine, Sendai, Japan
- Miura, Takahiro, Tohoku University Graduate School of Medicine, Sendai, Japan
- Yoichi, Sato, Tohoku University Graduate School of Medicine, Sendai, Japan
- Kohzuki, Masahiro, Tohoku University Graduate School of Medicine, Sendai, Japan
- Ito, Osamu, Tohoku Medical and Pharmaceutical University, Sendai, Japan
Background
Polycystic Kidney Disease (PKD) is a genetic disorder characterized by progressive enlargement of epithelial cysts, leading to destruction of renal function and which is a major cause of chronic kidney disease. We have previously shown that chronic exercise exerts beneficial effects including renal protection in hypertensive, diabetic and 5/6-nephrectomized rats. Therefore, we assessed the effects of moderate and high intensity chronic exercise on renal function in the PCK rat model of PKD.
Methods
Six-week-old, male PCK rats were divided into three groups: sedentary group (Sed, n=10), moderate exercise group (Exm, n=9) and high intensity exercise group (Exi, n=10). Exm and Exi underwent forced treadmill exercise for 12 weeks with the following protocols: 20m/min and 28m/min respectively for 60 min/day, 5 days/week. At the end of experiment, the blood and urine was collected and the kidney were removed. Cyst Area/Total Kidney Area ratio and podocyte injury index were examined.
Results
The present research showed the body weight (504.1±8.4 vs.431.8±7.9 and 433.6±4.3g) and total kidney weight (5.34±0.27 vs.4.22±0.41 and 4.20±0.06g) was significantly lower in both Exm and Exi compared with Sed after 12-week protocol of exercise. The kidney/body weight ratio had a declining tendency but not significant different between the groups. The urine volume(18.0±1.2 vs.13.1±0.9 and 13.0±0.8ml/day), urinary protein excretion (1426±120 vs.537±50 and 452±77mg/day) and blood urea nitrogen (18.2±0.9 vs.15.4±0.4 and 15.9±0.3mg/dL) also had remarkable decrease in both Exm and Exi compared with Sed. The result of serum creatinine had a declining tendency in both exercise groups. As demonstrated by the data, exercise alleviated cyst formation in PCK rats. The Cyst Area/Total Kidney Area ratio had significant decrease in the both exercise groups (30.2±3.4% vs.14.5±1.0% and 17.3±1.3%). The staining of desmin positive staining area in glomeruli as a marker of podocyte injury also had a descending tendency after the chronic exercise.
Conclusion
Both moderate and high intensity chronic exercise significantly decreased cystic area in PCK rats, which also have beneficial effects on attenuating the urinary protein excretion and preventing podocyte injury in this model of PKD.