Abstract: FR-PO282

MicroRNA Analysis in Secondary Hyperparathyroidism

Session Information

Category: Mineral Disease

  • 1202 Mineral Disease: Vitamin D, PTH, FGF-23

Authors

  • Kanai, Genta, Tokai University School of Medicine, Isehara, KANAGAWA, Japan
  • Nakamura, Michio, Tokai University School of Medicine, Isehara, KANAGAWA, Japan
  • Fukagawa, Masafumi, Tokai University School of Medicine, Isehara, KANAGAWA, Japan
Background

Hyperparathyroidism is characterized by decreased calcium receptor expression and cell proliferation. However, the mechanism of progression is not clear. MicroRNA (miRNA) is a short RNA that is not translated into protein and has regulatory function of gene expression. To investigate the role of miRNA in cell proliferation of the parathyroid gland, we performed comprehensive miRNA analysis using the next generation sequence.

Methods

The parathyroid gland removed from patients with renal failure was used for the experiment. miRNAs extracted from the largest gland and the smallest gland in the same individual glands were examined. When analyzing the sequence of more than 1 million leads, about 25% of them corresponded to about 2,600 known miRNAs.

Results

There were 71 kinds of sequences detected in more than 10,000 leads in any glands, and 98 kinds of sequences with more than 1000 leads and less than 10,000 leads. The non-enlarged parathyroid gland was compared with the largest gland, and miRNAs that upregulated more than 2-fold were 11 and 10, respectively. Furthermore, miRNAs downregulated more than 2-fold were 14 and 24 kinds, respectively. Up to 31-fold expression difference was observed in these sequences.

Conclusion

The miRNA profile showed differential expression by parathyroid size. This suggests that miRNA may regulate parathyroid cell proliferation.