Abstract: TH-PO045
Dietary Supplementation with Bacillus Superoxide Dismutase Protects against Contrast-Induced AKI in Mice
Session Information
- AKI: Epidemiology, Risk Factors, and Prevention - 1
October 24, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 101 AKI: Epidemiology, Risk Factors, and Prevention
Authors
- Choi, Young Eun, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Jang, Yookyung, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Yoon Sook, Ko, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Hee Young, Lee, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Koo, Tai yeon, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Oh, Sewon, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Kim, Myung-Gyu, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Jo, Sang-Kyung, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Yang, Jihyun, Kangbuk Samsung Hospital, Jongno-gu, Seoul, Korea (the Republic of)
Background
Superoxide dismutase (SOD) is a metalloenzyme that play an important role in antioxidant defense against oxidative stress. Oxidative stress was known as one of the main pathophysiology in contrast-induce acute kidney injury (CI-AKI). Based on recent data showing the important role of gut dysbiosis, using probiotics could have a potential to modify systemic oxidative stress. We investigated the renoprotective potential of orally administered enteric-coated SOD protein purified from Bacillus amyloliquefaciens (SOD-BA) in contrast-induced acute kidney injury (CI-AKI) in mice.
Methods
CI-AKI model was induced with unilateral nephrectomy using 5 week-old C57BL/6 male mice. Giving oral gavage SOD-BA once a day, 48 hours before intravenous contrast injection (10mg/kg) 4 weeks after unilateral nephrectomy. Serum blood urea nitrogen (BUN), creatinine, and kidney tissue NGAL were determined.
Results
SOD-BA did not have kidney toxicity in the vehicle group. The unilateral CI-AKI model showed minimal kidney function deterioration based on creatinine level. However, the serum BUN and NGAL were strikingly increased. SOD-BA oral administration group showed decreased BUN and kidney NGAL level.
Conclusion
Prophylactic treatment of oral SOD-BA could serve as a potent antioxidant in the prevention and treatment of CI-AKI.
Funding
- Commercial Support – Department of, Biomlogic/Genofocus