Abstract: SA-PO0601
Metabolomic Analysis of Renal Cyst Fluid in Patients with Advanced ADPKD
Session Information
- Cystic Kidney Diseases: Clinical Research
November 08, 2025 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Genetic Diseases of the Kidneys
- 1201 Genetic Diseases of the Kidneys: Monogenic Kidney Diseases
Authors
- Schueler, Jan, Universitat Regensburg, Regensburg, BY, Germany
- Heckscher, Simon, Universitat Regensburg, Regensburg, BY, Germany
- Ihlo, Nicolas A., Universitat Regensburg, Regensburg, BY, Germany
- Kellermeier, Fabian, Universitat Regensburg, Regensburg, BY, Germany
- Werner, Jens M., Universitatsklinikum Regensburg, Regensburg, BY, Germany
- Nuebel, Barbara, Universitatsklinikum Erlangen, Erlangen, BY, Germany
- Groß, Verena, Klinikum St Elisabeth Straubing GmbH, Straubing, BY, Germany
- May, Matthias, Klinikum St Elisabeth Straubing GmbH, Straubing, BY, Germany
- Wullich, Bernd, Universitatsklinikum Erlangen, Erlangen, BY, Germany
- Kammerl, Martin Christian, Technische Hochschule Deggendorf, Deggendorf, BY, Germany
- Gnewuch, Carsten, Universitatsklinikum Regensburg, Regensburg, BY, Germany
- Burkhardt, Ralph, Universitatsklinikum Regensburg, Regensburg, BY, Germany
- Buchholz, Bjoern, Universitat Regensburg, Regensburg, BY, Germany
- Pion, Eric, Universitat Regensburg, Regensburg, BY, Germany
- Banas, Miriam C., Universitatsklinikum Regensburg, Regensburg, BY, Germany
- Oefner, Peter J., Universitat Regensburg, Regensburg, BY, Germany
- Dettmer, Katja, Universitat Regensburg, Regensburg, BY, Germany
- Gronwald, Wolfram, Universitat Regensburg, Regensburg, BY, Germany
- Behr, Merle, Universitat Regensburg, Regensburg, BY, Germany
- Haerteis, Silke, Universitat Regensburg, Regensburg, BY, Germany
- Schmidt, Katharina Maria, Universitatsklinikum Regensburg, Regensburg, BY, Germany
Background
Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary kidney disorder. It is characterized by progressive renal cyst formation, often leading to end-stage kidney disease (ESKD) requiring kidney replacement therapy. In contrast to the urinary metabolome in ADPKD, the renal cyst fluid metabolome remains largely unexplored.
Methods
We conducted a comprehensive analysis of renal cyst fluid from 26 ADPKD patients (20 on hemodialysis, six with kidney transplants) by 1H-NMR spectroscopy, liquid chromatography-mass spectrometry (LC-MS), and clinical chemistry testing. Cysts were clustered based on metabolite profiles, and differences were analyzed across groups defined by renal function status (dialysis vs. transplant), cyst volume, and cyst sodium concentration.
Results
Dialysis patients and transplant recipients differed significantly in their cyst fluid metabolomes. The increased detection of creatinine in dialysis patients compared to transplant recipients is expected, as is the presence of metabolites of the prodrug mycophenolate mofetil in transplant patients. In addition, further differences were observed, including higher concentrations of myo-inositol, sucrose, τ-methylhistidine, trigonelline, and sarcosine in dialysis patients, whereas transplant recipients showed increased levels of leucine, isoleucine, valine, and alanine. Interindividual variability exceeded intraindividual metabolic differences between cyst fluids from the same patient, with cyst size not affecting metabolic profiles.
Conclusion
This first metabolomic analysis of renal cyst fluids in advanced ADPKD reveals distinct metabolic signatures linked kidney replacement therapy. The data provides novel insights into the pathophysiology of ADPKD and highlights the potential of renal cyst fluid metabolomics in the identification of biomarkers and therapeutic targets.
Funding
- Government Support – Non-U.S.