Abstract: TH-PO0147
Protective Effect and Mechanism of Qi Huang Pei Yuan Tang Combined with Reduced Glutathione on Acute Ischemia-Reperfusion Kidney Injury in Rats
Session Information
- AKI: Mechanisms - 1
November 06, 2025 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Ji, Manshan, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
- Luo, Luojiajin Jiajin, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
- Zhou, Zhu, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Background
Acute renal ischemia-reperfusion injury (R/I) is one of the most important causes of acute kidney injury (AKI) in clinical practice, and its core mechanisms include oxidative stress, inflammatory response and apoptosis.
Methods
Fifty healthy male SD rats were randomly divided into five groups: sham-operated group (Sham), model group (R/I), GSH-treated group (XY+R/I), Astragalus Peiyuan Tang group (ZY+R/I), and combined-treatment group (LH+R/I).The R/I model was established by clamping the bilateral renal arteries for 45 minutes, and serum and renal tissue samples were collected at 24 hours after surgery.
Statistical analysis: one-way ANOVA was performed using GraphPad Prism 9.5.0, and P<0.05 was considered significant.
Results
The results of the study showed that postoperative renal injury was significantly improved in the LH+R/I group of rats, as evidenced by a significant reduction in serum creatinine and urea nitrogen levels; inflammation levels and oxidative stress levels were significantly improved; pathological examination showed that renal tubular epithelial cell detachment and renal mesangial congestion were significantly attenuated in the LH+R/I group; and Western blot analysis showed that the expression of Nrf2, HO-1, and Western blot analysis showed that the expression of Nrf2, HO-1 and GPX4 proteins was significantly up-regulated in the combined treatment group, which further verified that the combined treatment played a protective role by regulating the Nrf2/HO-1 pathway to alleviate oxidative stress.
Conclusion
The combined treatment effectively improved renal function and attenuated acute ischemia-reperfusion kidney injury in rats. This study provides a new strategy for combining Chinese and Western medicine in the treatment of acute kidney injury, suggesting that the combination of Chinese medicine compound and antioxidants has the potential for multi-target synergistic effects.
Funding: This work was supported by Yunnan Major Scientific and Technological Project, (grant no. 202102AA100060), Yunnan Province's Xingdian Talents Program for Renowned Physicians Support Plan.
Funding
- Other NIH Support