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Kidney Week

Abstract: FR-PO0361

Inflammation Cytokines and Adipokines in Patients with Diabetes and Obesity with and Without CKD

Session Information

Category: Diabetic Kidney Disease

  • 702 Diabetic Kidney Disease: Clinical

Authors

  • Mahajan, Sandeep, All India Institute of Medical Sciences New Delhi, New Delhi, DL, India
  • Subbiah, Arunkumar, All India Institute of Medical Sciences New Delhi, New Delhi, DL, India
  • Rizwan, Arshi, All India Institute of Medical Sciences New Delhi, New Delhi, DL, India
Background

Epidemic of diabetes & obesity is fueling increasing cases of CKD worldwide. Mechanisms involved in complex interplay between obesity, diabetes and CKD are poorly studied, though inflammation & shift in adipokine and cytokine production towards pro-inflammatory state have been implicated
We in this cross-sectional, case control study looked at HsCRP & important pro-inflammatory (leptin, IL-6, TNF-α) & anti-inflammatory mediators (adiponectin, IL-10) in subjects with obesity alone, obesity with diabetes & obesity with diabetes & CKD & compared it with healthy controls

Methods

60 consenting subjects in each group were studied. Besides detailed history, BMI calculation; serum levels of HsCRP, adipokines (leptin & adiponectin) & cytokines (IL-6, TNF-α & IL-10) were assessed using commercially available ELISA kits

Results

Table shows demographic, clinical & study parameters of each group. Patient groups were gender matched but slightly older than controls. Obese subjects in various groups had similar BMI & waist/hip ratio. All obese groups had evidence of pro-inflammatory state as evidenced by higher HsCRP, leptin, IL6 & TNF-α than controls, with obese diabetic patients with CKD showing maximum aberrations. Levels of adiponectin were lower only in patients with obesity with complications of DM & CKD while IL-10 levels were higher in these groups

Conclusion

We document higher pro-inflammatory milieu in obese subjects, which worsens progressively with complications of DM & CKD. Though, absolute values of anti-inflammatory mediators are not affected to similar extent but they might be relatively impaired considering overall pro-inflammatory state

Demographic clinical & study parameters
ParameterControlsObese aloneObese with DMObese with DM & CKD
Age (yr)43.1 ± 7.545.5 ± 6.4*50.4±10.5*51.3±12.2*
Male (%)61.66053.360
BMI22.7±2.131.8±1.4*31.4±2.1*30.4±1.8*
Waist/Hip ratio0.8±.10.9±0.2*0.9±0.1*1.0±0.1*
eGFR (ml/min)108.3±26.4102.7±20.596.6±9.7*^40.0±10.2*^#
HsCRP (mg/L)1.1±0.12.4±0.4*3.9±0.2*^$8.4±0.1*^#
Leptin (ng/ml)3.7±0.258.6±5.2*62.3±17.2*$83.4±12.2*^#
Adiponectin (pg/ml)5.3±0.75.5±0.33.7±0.9*^2.9±0.6*^
IL-6 (pg/ml)72.4±8.283.4±8.4*174.5±10.3*^$392.9±22.2*^#
TNF-α (pg/ml)5.4±1.17.8±1.2*11.3±0.8*^$21.6±1.9*^#
IL-10 (pg/ml)101.8±14.271.4±11.9*139.1±16.6*^131.1±36.1*^

*p<0.05 vs group 1, ^ p<0.05 vs group 2, # p<0.05 vs group 3, $ p<0.05 vs group 4

Digital Object Identifier (DOI)