Abstract: SA-PO0414
Human Umbilical Cord Mesenchymal Stem Cells Exert Protective Effects in Peritoneal Fibrosis via cGAS-STING Signaling Pathway
Session Information
- Home Dialysis: Science and Cases, from Lab to Living Room
November 08, 2025 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 802 Dialysis: Home Dialysis and Peritoneal Dialysis
Author
- Dong, Fuxing, Fujian Provincial Hospital, Fuzhou, Fujian, China
Background
Clinical evidence indicates exposure of the peritoneum to non-physiological peritoneal dialysis fluid may result in peritoneal fibrosis(PF).MSCs can repair damage and fibrosis in organs.In our recently research,we have estimated CAS-STING signal transmissoin is closely associated with PF.
Methods
HG-induced HMrSV5 EMT and mouse peritoneum fibrosis induced by PF. hUC-MSCs were co-cultured with HMrSV5, and their effects on HG-induced EMT were evaluated using wound healing, Transwell migration, qPCR, and WB,IHC. Role of cGAS-STING pathway was confirmed using the STING agonist DMXAA.
Results
cGAS and STING are upregulated in HG-induced HMrSV5 EMT and mouse PF. Co-culture of hUC-MSCs with HMrSV5 suppresses HMrSV5 EMT induced by HG via inhibition of cGAS-STING signaling pathway. Moreover, treatment of PF mice with hUC-MSCs alleviates PF,local inflammation,downregulates proteins of the cGAS-STING signaling pathway ,
Conclusion
hUC-MSCs mitigate EMT in HMrSV5 and retard the progression of PF in mice through inhibition of the cGAS-STING signaling pathway.
Fig.1. hUC-MSCs suppress HG induced PF,HMrSV5 EMT ,cgas and sting expression.Data are presented as the mean±SD. *P <0.05 vs. Control, #P <0.05 vs. HG.
Fig.2. hUC-MSCs suppress HG induced EMT in HMrSV5 by inhibiting cGAS-STING signaling pathway.Data are presented as the mean±SD. *P <0.05 vs. Control, #P <0.05 vs. DMXAA.
Funding
- Government Support – Non-U.S.