Abstract: TH-PO0140
Natural Killer T Cells in Acute Cardiorenal Syndrome
Session Information
- AKI: Mechanisms - 1
November 06, 2025 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Burfeind, Kevin G., Oregon Health & Science University, Portland, Oregon, United States
- Aomura, Daiki, Oregon Health & Science University, Portland, Oregon, United States
- Hebert, Jessica Faith, Oregon Health & Science University, Portland, Oregon, United States
- Groat, Tahnee, Portland VA Medical Center, Portland, Oregon, United States
- Hutchens, Michael, Portland VA Medical Center, Portland, Oregon, United States
Group or Team Name
- Hutchens Lab.
Background
Acute cardiorenal syndrome is a common cause of acute kidney injury that often rapidly transitions to chronic kidney disease. Natural killer T-cells (NKT) are tissue resident T-cells that have not been investigated in acute cardiorenal syndrome.
Methods
We modeled acute cardiorenal syndrome with cardiac arrest and cardiopulmonary resuscitation (CA/CPR) in mice. CA was induced with potassium chloride and CPR with epinephrine and chest compressions (Fig. 1A). NKTs were depleted with intravenous injection of anti-NK1.1 antibody or genetically ablated in CD1d1 knockout (CD1dKO) mice. Kidney function was assessed by glomerular filtration rate (GFR) and urine output 24 hours after CA/CPR. Single cell RNA sequencing was performed on whole kidney and isolated kidney NKT cells.
Results
NK1.1 antibody administration and CD1dKO depleted NKTs and caused increased GFR and urine output (Fig. 1F/G) after CA/CPR. NK1.1 antibody administration caused decreased mRNA markers of proximal tubule (PT) injury. PT dedifferentiation was reduced by CD1dKO (Fig. 2B). NKT cells were more cytotoxic after CA/CPR (Fig. 2C/D).
Conclusion
NKT depletion ameliorated PT dedifferentiation and kidney injury in acute cardiorenal syndrome, suggesting NKTs may contribute to PT dedifferentiation and injury.
Funding
- Veterans Affairs Support