Abstract: TH-PO0445
Immunomodulatory Effects of Single Hemodialysis: A Single-Cell Transcriptomic Approach
Session Information
- Hemodialysis: Novel Markers and Case Reports
November 06, 2025 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 801 Dialysis: Hemodialysis and Frequent Dialysis
Authors
- Kwon, Jin Kyung, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Oh, Jae-ik, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea (the Republic of)
- Lee, Seong Min, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Ku, Hyunah, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea (the Republic of)
- Lee, Jinsun, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea (the Republic of)
- Kim, Dong Ki, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Yang, Seung Hee, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Park, Sehoon, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
Background
End stage kidney disease (ESKD) is an irreversible complication of chronic kidney disease (CKD) and hemodialysis (HD) is known as primary alternative option for ESKD patient. While chronic impairment of immunity in maintenance HD patients is well known, the acute effects of HD on immunity are not fully understood. This study aims to verify the acute effect of single hemodialysis on patients’ immunity by analyzing patient's peripheral blood mononuclear cell (PBMC) by single-cell RNA sequencing (scRNA-seq).
Methods
Three males and five females receiving maintenance HD were enrolled in this study. The mean age and mean dialysis vintage of study population were 61.8 years and 155.4 months, respectively. PBMC were earned from the blood sample taken immediately before HD and after HD. The PBMC cells were sequenced by 10x Genomics, and were processed by CellRanger and Seurat. 92,102 cells were obtained, and 66,234 were checked after the quality control and doublet-removal. The sequenced data were clustered by PBMC cell type, and differentially expressed gene (DEG) analyses with pseudobulking were performed using DESqe2.
Results
The proportion of CD4+ naive T cell, CD4+ central memory T cell, CD8+ naive T cell, CD8+ effector memory T cell, naive B cell, intermediate B cell, and regulatory T cell were increased after HD, whereas CD14+ monocyte and conventional dendritic cell were decreased. In DEG analysis, genes related with immune response regulation including Toll-like receptor (TLR)-4 cascade were downregulated in naive B cell. In CD4+ T cells, genes related with cytokine interaction and chemokine-mediated signaling were downregulated. TNFRSF10B, which is related with TRAIL pathway, was upregulated in CD8+ naive T cell.
Conclusion
This study suggests that a single session of HD may affect patients’ immune systems at the gene expression level. Our study may provide future potential target for reconstituting immune systems of patients undergoing maintenance HD.
UMAP plot showing PBMC clusters from scRNA-seq data