ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on X

Kidney Week

Abstract: FR-PO0112

Next Extracorporeal Albumin Detoxification/Dialysis (NxECAD) Based on Purified Albumin Dialysate, but Not MARS, Reverses Leukocyte Dysfunction Due to Cholestasis in Multiorgan Failure

Session Information

Category: Acute Kidney Injury

  • 102 AKI: Clinical, Outcomes, and Trials

Authors

  • Stange, Jan, Dept. Internal Medicine, Rostock, MV, Germany
  • Koball, Sebastian, Dept. Internal Medicine, Rostock, MV, Germany
  • Hinz, Michael, Dept. Internal Medicine, Rostock, MV, Germany
  • Mitzner, Steffen R., Dept. Internal Medicine, Rostock, MV, Germany

Group or Team Name

  • Opalesce Team.
Background

Albumin bound toxins (ABT) contribute to renal, -multiorgan failure, and immune paralysis. On top of providing renal support therapy, one goal of extracorporeal albumin detoxification/dialysis (ECAD) is to remove ABT to accelerate resolution of secondary organ failures. Recent research has revealed that elevated levels of certain bile acids due to cholestasis cause leucocyte dysfunction. The aim of this study was to compare the effect of extracorporeal Albumin Dialysis (ECAD) using MARS and NxECAD employing enhanced efficacy charcoal for dialysate albumin recycling, on patient’s leucocyte function.

Methods

In an RCT, subjects with progressive jaundice and/or HE and/or RF and/or PR were treated first with one ECAD (MARS or NxECAD) and afterwards crossed over to the other. Pre-and post-values of markers for ABT, Safety and other clinical parameters were compared. Pre- and post-treatment plasma samples were tested for efficacy of ABT removal by Albumin Binding Functions (ABF) using two different methods (dansylsarcosine binding and dodecanoic acid binding by Electron Spin Resonance-ESR-Spectroscopy), concentrations of Bile Acids and suppressive effects on leucocyte function.

Results

The percentage of phagocytotic granulocytes remained unchanged when samples before and after MARS were compared. However, it increased significantly when samples pre-NxECAD were compared to samples post NxECAD. Total Bile Acids were reduced significantly by MARS and NxECAD, the change from baseline (CFBL) during NxECAD was significantly higher than for MARS. In parallel, patient`s Albumin Binding Function increased significantly during NxECAD treatments, but not during MARS. Analysis of granulocyte phagocytosis confirmed a reduction due to the disease which could NOT be reversed by MARS, but significantly recovered during NxECAD.

Conclusion

Since ECAD appears to have an efficacy dependent effect on reversing leucocyte dysfunction related to accumulation of albumin bound toxins, further clinical studies should investigate if these laboratory findings translate into a reduction of infection rates, improvement of antibiotic response and ultimately survival in subjects with liver failure on extracorporeal support.

Funding

  • Government Support – Non-U.S.

Digital Object Identifier (DOI)