Kidney Week

Abstract: TH-PO651

Mechanisms Involved in the Disturbances in Renal Development in Pups from Vitamin D Deficient Dams

Session Information

• Development, Stem Cells, Regenerative Medicine - I
  October 25, 2018 | Location: Exhibit Hall, San Diego Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Development, Stem Cells, and Regenerative Medicine

• 501 Development, Stem Cells, and Regenerative Medicine: Basic

Authors

• Almeida, Lucas Ferreira, University of Sao Paulo, Ribeirao Preto, Brazil

Lucas Ferreira Almeida,

• Francescato, Heloisa Della coletta, University of Sao Paulo, Ribeirao Preto, Brazil

Heloisa Della coletta Francescato,

• Silva, Cleonice, Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto, Brazil

Cleonice Silva,

• Rodrigues, Jose Antunes, FMRP-USP, Ribeirão Preto, Brazil

Jose Antunes Rodrigues,

• Coimbra, Terezila Machado, University of Sao Paulo, Ribeirao Preto, Brazil

Terezila Machado Coimbra,

Background

Vitamin D₃ (Vit.D₃) has been related with cellular proliferation, differentiation and apoptose and with the regulation of renin gene, which are important events in renal development. Pups from dams submitted to a Vit.D₃ deficient diet during nephrogenesis (gestation and lactation), present changes in renal development that can persist in adult life. This study investigates the influence of Vit.D₃ deficiency on changes in renal development, the mechanisms involved and its consequences in adult life.

Methods

Offspring (Hannover rats) from mothers fed with normal (control group, CG) or vitamin D restricted (VitD-) diets were used in this study. Body weight (BW) was evaluated at birth, during lactation and at 3 months and 6 months of age. Systolic blood pressure (SBP) was measured monthly using a tail-cuff plethysmography. Blood and urine samples were collected to quantify Vit.D3, creatinine, Na⁺, K⁺, Ca⁺², angiotensin II (ANGII) and albuminuria levels. The kidneys were removed for histological, morphometric and immunohistochemical studies 3 and 6 months after birth.

Results

Vit.D deficient pups presented decreased BW at the end of lactation and increase in adult life compared to CG (p< 0.05). These animals showed higher SBP and plasma ANGII levels in the adult life compared to CG (p< 0.05). These pups also presented a significant decrease in urine osmolality, sodium and potassium fractional excretion and increase in water intake and urinary volume (p< 0.05). Decreased expressions of JG12 (a marker of endothelial cells) in renal cortex and glomerulus, and of synaptopodin (a marker of filtration barrier) in glomerulus were observed in VitD- compared to CG (p< 0.05). These animals also presented decreased glomerular area and increased fractional mesangial area compared to CG (p< 0.05). However, there was no difference in serum calcium levels, glomerular filtration rate, and albuminuria between the groups.

Conclusion

The current findings showed that the renal development is impaired in pups from dams VitD restricted with consequences in their adult life. These alterations can be at least in part provoked by the disturbances in the renal angiogenesis induced by the renin angiotensin system upregulation in pups with Vit.D deficiency

Funding

• Government Support - Non-U.S.