Abstract: TH-PO756
Mussel-Inspired Approach Towards Heparin-Immobilized Cellulose Hydrogel for Selective Removal of Low Density Lipoprotein from Whole Blood
Session Information
- Bioengineering
October 25, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Bioengineering
- 300 Bioengineering
Authors
- Li, Yupei, West China Hospital, Sichuan University, Chengdu, Sichuan, China
- Su, Baihai, West China Hospital, Sichuan University, Chengdu, Sichuan, China
- Zhao, Weifeng, College of Polymer Science & Engineering, Sichuan University, Chengdu, China
- Xiong, Yuqin, West China Hospital, Sichuan University, Chengdu, Sichuan, China
- Liao, Ruoxi, West China Hospital, Sichuan University, Chengdu, Sichuan, China
- Wang, Yilin, College of Polymer Science & Engineering, Sichuan University, Chengdu, China
Background
Cardiovascular disease is the leading cause of death in most countries and is responsible for a significant economic and health care burden. To date, it has been well established that the elevated low density lipoprotein (LDL) level in serum is associated with the development and progression of coronary artery disease, stroke, heart failure and peripheral arterial disease. Although statins exhibit excellent efficacy of lowering LDL in most occasions, they fail to improve the outcomes of patients with familial hypercholesterolemia without LDL apheresis. Dextran sulfate adsorption and heparin-induced extracorporeal LDL precipitation are commercially available at present in the US. However, both of them require initial separation of plasma, making it impossible to remove LDL from whole blood. Therefore, it is of great importance to develop a new LDL absorbent to overcome the drawbacks of the existing techniques.
Methods
Heparin-immobilized cellulose (HeTaCe) hydrogels were fabricated through a mussel-inspired approach. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, thermo-gravimetric analysis were used to characterize the chemical compositions of the hydrogels. The contact activation, complement activation, hemolysis ratio and clotting times of the hydrogels were performed to investigate the blood compatibility. Moreover, we conducted both statical adsorption test and dynamic simplified simulative column adsorption test to evaluate the adsorption clearance, capacity and selectivity of LDL from whole blood in vitro.
Results
Characterization of the hydrogels confirmed the successful synthesis of the new absorbent. HeTaCe hydrogels exhibited low hemolysis ratio (<1%), suppressed complement activation and contact activation, mild impact on whole blood and prolonged clotting times. In vitro, an ideal adsorption capacity of 79.1 mg/g with a significant clearance of 48.3% from 14.77 to 7.64mmol/L towards LDL was achieved and the dynamic adsorption test further demonstrated a selective adsorption of LDL without a significant reduction of HDL level in a simulative system.
Conclusion
HeTaCe hydrogels may have great potential application in safe and efficient LDL adsorption from whole blood, making it possible to improve the outcomes of patients with high cardiovasular risk.