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Abstract: SA-PO609

Influence of Renal Ischemia-Reperfusion Injury on Renal Neutrophil Gelatinase-Associated Lipocalin Receptor (24p3R) in Rats

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Arakawa, Yusuke, Nippon Medical School, Tokyo, Bunkyo, Japan
  • Ushijima, Kentarou, Jichi Medical University, Shimotsuke, Japan
  • Mii, Akiko, Nippon Medical School, Tokyo, Japan
  • Tsuruoka, Shuichi, Nippon Medical School, Tokyo, Japan
  • Fujimura, Akio, Jichi Medical Univ., Simotsuke, Japan
Background

The neutrophil gelatinase-associated lipocalin (NGAL) receptor (24p3R) is expressed in distal nephron, and contributes to the endocytosis of NGAL in urine. This study was undertaken to evaluate an influence of renal ischemia-reperfusion injury on 24p3R.

Methods

Unilateral renal pedicle was clamped for 0, 10, 20, 30, or 45 min in male Wistar rats. Urine was collected for 24hours after reperfusion, and ischemic kidney was obtained.

Results

Renal NGAL mRNA expression and urinary NGAL excretion elevated in rats with renal ischemia for more than 20min. On the other hand, renal mRNA expressions of 24p3R, and megalin and cubilin which are expressed in proximal nephron and uptake NGAL in urine, decreased in animals with renal ischemia for more than 20min. Renal protein expressions of 24p3R and megalin decreased in rats with renal ischemia for 30 and 45min.

Conclusion

This study showed for the first time that renal 24p3R decreased in response to renal ischemia. As NGAL in urine is reabsorbed by the megalin-cubilin complex at the proximal nephron and by the 24p3R at the distal nephron, the down-regulations of these proteins might contribute to the elevated urinary NGAL excretion in rats with renal ischemia-reperfusion injury.